Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Japan (Japanese)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      Purified Mouse Anti-Human Cyclin E

      BD Pharmingen™ Purified Mouse Anti-Human Cyclin E

      (RUO)
      Purified Mouse Anti-Human Cyclin E
      Left Figure: Western blot analysis of Cyclin E expression on HeLa cell lysate. Lysate from HeLa cells (ATCC CCL-2) was probed with Purified Mouse Anti-Human Cyclin E antibody (Cat. No. 551160/551159) at concentrations of 1.0 µg/mL (lane 1), 0.5 µg/mL (lane 2), and 0.25 µg/mL (lane 3). Cyclin E is identified as a band of 50 kDa. Right Figure: Immunofluorescent analysis of Cyclin E expression on HeLa cells. HeLa cells were seeded in a 96-well imaging plate at ~10,000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and Purified Mouse Anti-Human Cyclin E antibody. The second step reagent was Alexa Fluor® 555 goat anti-mouse IgG (Invitrogen). The image was taken on a BD Pathway™ 855 Bioimager system using a 20x objective.  This antibody also stained A549 (ATCC CCL-185) and U-2 OS (ATCC HTB-96) cells and worked with both the Triton™ X-100 and alcohol perm protocols (see Recommended Assay Procedure).
      Purified Mouse Anti-Human Cyclin E
      Left Figure: Western blot analysis of Cyclin E expression on HeLa cell lysate. Lysate from HeLa cells (ATCC CCL-2) was probed with Purified Mouse Anti-Human Cyclin E antibody (Cat. No. 551160/551159) at concentrations of 1.0 µg/mL (lane 1), 0.5 µg/mL (lane 2), and 0.25 µg/mL (lane 3). Cyclin E is identified as a band of 50 kDa. Right Figure: Immunofluorescent analysis of Cyclin E expression on HeLa cells. HeLa cells were seeded in a 96-well imaging plate at ~10,000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and Purified Mouse Anti-Human Cyclin E antibody. The second step reagent was Alexa Fluor® 555 goat anti-mouse IgG (Invitrogen). The image was taken on a BD Pathway™ 855 Bioimager system using a 20x objective.  This antibody also stained A549 (ATCC CCL-185) and U-2 OS (ATCC HTB-96) cells and worked with both the Triton™ X-100 and alcohol perm protocols (see Recommended Assay Procedure).
      製品詳細
      Down Arrow Up Arrow


      BD Pharmingen™
      Human (QC Testing)
      Western blot (Routinely Tested), Bioimaging (Tested During Development), Flow cytometry (Reported)
      RUO
      AB_394080


      説明

      Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle.  These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small proteins that bind to cyclins, cdks, or cyclin-cdk complexes, e.g., p21 and p27 [Kip1].  Cyclin E is expressed in G1 and associates with cdk2 to form an active kinase where it plays an important role in the regulation of the G1/S restriction checkpoint in the cell cycle. Abberant expression of cyclin E has been reported to be associated with the oncogenic transformation of cells.  This antibody has been reported not to cross-react with mouse cyclin E.

      推奨アッセイ手順

      Bioimaging

      1. Seed the cells in appropriate culture medium at ~10,000 cells per well in a 96-well Imaging Plate and culture overnight.

      2. Remove the culture medium from the wells, and fix the cells by adding 100 μl of BD Cytofix™ Fixation Buffer (Cat. No. 554655) to each well.  Incubate for 10 minutes at room temperature (RT).

      3. Remove the fixative from the wells, and permeabilize the cells using either BD Perm Buffer III or Triton™ X-100:

      a. Add 100 μl of -20°C Perm Buffer III (Cat. No. 558050) to each well and incubate for 5 minutes at RT.

           OR

      b. Add 100 μl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.

      4. Remove the permeabilization buffer, and wash the wells twice with 100 μl of 1× PBS.

      5. Remove the PBS, and block the cells by adding 100 μl of BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656) to each well. Incubate for 30 minutes at RT.

      6. Remove the blocking buffer and add 50 μl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.

      7. Remove the primary antibody, and wash the wells three times with 100 μl of 1× PBS.

      8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 μl to each well, and incubate in the dark for 1 hour at RT.

      9. Remove the second step reagent, and wash the wells three times with 100 μl of 1× PBS.

      10. Remove the PBS, and counter-stain the nuclei by adding 200 μl per well of 2 μg/ml Hoechst 33342 (e.g., Sigma-Aldrich Cat. No. B2261) in 1× PBS to each well at least 15 minutes before imaging.

      11. View and analyze the cells on an appropriate imaging instrument.

      Bioimaging:  For more detailed information please refer to "Cellular Imaging" at our website: http://www.bdbiosciences.com/us/s/resources

      Western blot:  For more detailed information please refer to "Cell biology (WB, IP, IHC, IF)" at our website: http://www.bdbiosciences.com/us/s/resources

      詳細を表示 blue down arrow 表示項目を減らす blue up arrow

      製品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
      3. Triton is a trademark of the Dow Chemical Company.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      詳細を表示 blue down arrow 表示項目を減らす blue up arrow
      551159 Rev. 8
      構成品
      Down Arrow Up Arrow
      説明 数量/容量 Part Number EntrezGene ID
      Purified Mouse Anti-Human Cyclin E 50 µg (1 ea) 51-1459GR N/A
      HeLa Control Lysate 50 µg (1 ea) 51-16516N N/A
      551159 Rev. 8
      引用＆参考文献
      Down Arrow Up Arrow

      開発者向け参考資料 (11)

      1. Darzynkiewicz Z, Gong J, Juan G, Ardelt B, Traganos F. Cytometry of cyclin proteins. Cytometry. 1996; 25(1):1-13. (Biology: Flow cytometry).
      2. Faha B, Harlow E, Lees E. The adenovirus E1A-associated kinase consists of cyclin E-p33cdk2 and cyclin A-p33cdk2. J Virol. 1993; 67(5):2456-2465. (Biology: Western blot). 参考文献を見る
      3. Gong J, Bhatia U, Traganos F, Darzynkiewicz Z. Expression of cyclins A, D2 and D3 in individual normal mitogen stimulated lymphocytes and in MOLT-4 leukemic cells analyzed by multiparameter flow cytometry. Leukemia. 1995; 9(5):893-899. (Biology: Flow cytometry). 参考文献を見る
      4. Gong J, Li X, Traganos F, and Darzynkiewicz Z. Expression of G1 and G2 cyclins measured in individual cells by multiparameter flow cytometry: a new tool in the analysis of the cell cycle. Cell Prolif. 1994; 27:357-371. (Biology: Flow cytometry).
      5. Gong J, Traganos F, Darzynkiewicz Z. Simultaneous analysis of cell cycle kinetics at two different DNA ploidy levels based on DNA content and cyclin B measurements. Cancer Res. 1993; 53(21):5096-5099. (Biology: Flow cytometry).
      6. Gong J, Traganos F, Darzynkiewicz Z. Threshold expression of cyclin E but not D type cyclins characterizes normal and tumour cells entering S phase. Cell Prolif. 1995; 28(6):337-346. (Biology: Flow cytometry). 参考文献を見る
      7. Gong J, Traganos F, and Darzynkiewicz Z. Expression of cyclins B and E in individual MOLT-4 cells and in stimulated human lymphocytes during progression through the cell cycle. Int J Oncol. 1993; 3:1037-1042. (Biology: Flow cytometry).
      8. Gorospe M, Liu Y, Xu Q, Chrest FJ, Holbrook NJ. Inhibition of G1 cyclin-dependent kinase activity during growth arrest of human breast carcinoma cells by prostaglandin A2. Mol Cell Biol. 1996; 16(3):762-770. (Biology: Western blot).
      9. Keyomarsi K, O'Leary N, Molnar G, Lees E, Fingert HJ, Pardee AB. Cyclin E, a potential prognostic marker for breast cancer. Cancer Res. 1994; 54(2):380-385. (Biology: Western blot). 参考文献を見る
      10. Scuderi R, Palucka KA, Pokrovskaja K, Bjorkholm M, Wiman KG, Pisa P. Cyclin E overexpression in relapsed adult acute lymphoblastic leukemias of B-cell lineage. Blood. 1996; 87(8):3360-3367. (Biology: Western blot).
      11. Sherr CJ. Mammalian G1 cyclins. Cell. 1993; 73(6):1059-1065. (Biology). 参考文献を見る
      すべて表示する (11) 表示項目を減らす
      551159 Rev. 8

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback