FITC Mouse Anti-Human CD33
Clone P67.6 (RUO (GMP))
- Concentration 3 μg/mL
- Vol. Per Test 20 μL
- Isotype Mouse BALB/c IgG1, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen CD33-transfected FMY9S5 Cells
- Workshop No. IV M503
- Entrez Gene ID 945
- Storage Buffer Phosphate buffered saline with gelatin and 0.1% sodium azide.
- Regulatory Status RUO (GMP)
Regulatory Status Legend
CD33, clone P67.6, is derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with FMY9S5 cells containing the CD33 gene.
CD33 recognizes a human myelomonocytic antigen, Mr 67 kilodaltons (kd).
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
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Preparation and Storage
The monoclonal antibody is supplied as 12 μg purified immunoglobulin in 2.0 mL (6 μg/mL) of phosphate-buffered saline (PBS). The FITC conjugate is supplied as 3 μg in 1.0 mL (3 μg/mL) of PBS. The PE conjugate is supplied as 24 μg in 2.0 mL (12 μg/mL) of PBS. The PerCP-Cy5.5 conjugate is supplied as 6.3 μg in 1.0 mL (6.3 μg/mL) of PBS. The PE-Cy7 conjugate is supplied as 12.5 μg in 0.5 mL (25 μg/mL) of PBS. The APC conjugate is supplied as 6.2 μg in 0.5 mL (12.4 μg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide.
Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.