FITC Mouse Anti-Human CD27
Clone L128 (RUO (GMP))
- Concentration 0.8 μg/mL
- Vol. Per Test 20 μL
- Isotype Mouse BALB/c IgG1
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Human Activated Peripheral Blood Cells
- Workshop No. VI T6T037
- Entrez Gene ID 939
- Storage Buffer Phosphate buffered saline with gelatin and 0.1% sodium azide.
- Regulatory Status RUO (GMP)
Regulatory Status Legend
The CD27 antibody, clone L128, is derived from hybridization of mouse Sp2/0 cells with spleen cells from BALB/c mice immunized with activated peripheral blood lymphocytes.
The CD27 antibody recognizes a 110–120-kilodalton (kDa) disulfide-linked homodimer comprised of two 55-kDa polypeptide chains. The CD27 antigen is a lymphocyte-specific member of the tumor necrosis factor receptor (TNFR) super family, which also includes nerve growth factor receptor, CD30, CD40, CD95 (Fas), CD120a, CD120b, CD134 (OX 40), and CD137. The CD27 antigen is also known as S152, T14, TNFRSF7, and Tp55.
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
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Preparation and Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.