North America
South America
Australia & New Zealand
Europe
Middle East / Africa
Asia / Pacific
×
×
Purified Rat Anti-Mouse CD43 S7 RUO

Purified Rat Anti-Mouse CD43 

Clone  S7   (RUO)

  • Brand BD Pharmingen™
  • Alternative Name Spn; Sialophorin; Leukosialin; Ly-48; Ly48; Galgp; LEUK
  • Concentration 0.5 mg/ml
  • Isotype Rat DA x LOU IgG2a, κ
  • Reactivity Mouse (QC Testing) 
  • Application Flow cytometry (Routinely Tested) 
    (Co)-stimulation, Western blot (Reported) 
  • Immunogen Mouse Plasmacytoma MOPC-315
  • Entrez Gene ID 20737 
  • Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The S7 monoclonal antibody specifically binds to the 115 kDa glycosylated form of CD43 (Ly-48, Leukosialin). CD43 is expressed on IL-7-responsive pro-B cells, plasma cells, peritoneal and splenic CD5+ B cells (B-1 cells), granulocytes, monocytes, macrophages, platelets, natural killer cells, thymocytes, peripheral T cytotoxic/suppressor cells, and most T helper cells, but not resting conventional peripheral B cells. CD43 expression has also been detected on pluripotent hematopoietic stem cells and myeloid, lymphoid, and NK-cell progenitors in the bone marrow. Studies of CD43-deficient mice indicate that CD43 participates in the negative regulation of T-cell activation and adhesion.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Format
  • Format Purified

Other Formats →

Suggested Companion Products

FITC Goat Anti-Rat Ig Polyclonal RUO
0.5 mg Cat No: 554016

Purified Rat IgG2a, κ Isotype Control RUO
0.5 mg Cat No: 553927

Resources & Tools
 Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.


  1. Gulley ML, Ogata LC, Thorson JA, Dailey MO, Kemp JD. Identification of a murine pan-T cell antigen which is also expressed during the terminal phases of B cell differentiation. J Immunol. 1988; 140(11):3751-3757. View reference

  2. Hardy R, Hayakawa K. Generation of Ly-1 B cells from developmentally distinct precursors. Enrichment by stromal-cell culture or cell sorting. Ann N Y Acad Sci. 1992; 651:99-111. View reference

  3. Hardy RR, Carmack CE, Shinton SA, Kemp JD, Hayakawa K. Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow. J Exp Med. 1991; 173(5):1213-1225. View reference

  4. Jones AT, Federsppiel B, Ellies LG, et al. Characterization of the activation-associated isoform of CD43 on murine T lymphocytes. J Immunol. 1994; 153(8):3426-3439. View reference

  5. Manjunath N, Correa M, Ardman M, Ardman B. Negative regulation of T-cell adhesion and activation by CD43. Nature. 1995; 377(6549):535-538. View reference

  6. Moore T, Bennett M, Kumar V. Transplantable NK cell progenitors in murine bone marrow. J Immunol. 1995; 154(4):1653-1663. View reference

  7. Moore T, Huang S, Terstappen LW, Bennett M, Kumar V. Expression of CD43 on murine and human pluripotent hematopoietic stem cells. J Immunol. 1994; 153(11):4978-4987. View reference

  8. Rolink A, ten Boekel E, Melchers F, Fearon DT, Krop I, Andersson J. A subpopulation of B220+ cells in murine bone marrow does not express CD19 and contains natural killer cell progenitors. J Exp Med. 1996; 183(1):187-194. View reference

  9. Sperling AI, Green JM, Mosley RL, et al. CD43 is a murine T cell costimulatory receptor that functions independently of CD28. J Exp Med. 1995; 182(1):139-146. View reference

  10. Stockton BM, Cheng G, Manjunath N, Ardman B, von Andrian UH. Negative regulation of T cell homing by CD43. Immunity. 1998; 8(3):373-381. View reference

  11. Thurman EC, Walker J, Jayaraman S, Manjunath N, Ardman B, Green JM. Regulation of in vitro and in vivo T cell activation by CD43. Int Immunol. 1998; 10(5):691-701. View reference

  12. Wells SM, Kantor AB, Stall AM. CD43 (S7) expression identifies peripheral B cell subsets. J Immunol. 1994; 153(12):5503-5515. View reference

  13. Woodman RC, Johnston B, Hickey MJ, et al. The functional paradox of CD43 in leukocyte recruitment: a study using CD43-deficient mice. J Exp Med. 1998; 188(11):2181-2186. View reference

553268 Rev. 16
Instruments
Reagents
Applications
Sales & Support
BD BioSciences