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      FITC Mouse Anti-Human Syk
      FITC Mouse Anti-Human Syk
      Flow cytometric analysis of Syk. Raji cells (1 x 10e6 cells/ml) were fixed and permeabilized using BD Cytofix/Cytoperm™ (30 minutes at room temperature). Cells were then stained (1 µg/ml for 1 hr at RT) with either a FITC-conjugated mouse Syk antibody (Cat. No. 552476) or a FITC conjugated mouse isotype control. Cells were washed and analyzed by flow cytometry.
      FITC Mouse Anti-Human Syk
      Flow cytometric analysis of Syk. Raji cells (1 x 10e6 cells/ml) were fixed and permeabilized using BD Cytofix/Cytoperm™ (30 minutes at room temperature). Cells were then stained (1 µg/ml for 1 hr at RT) with either a FITC-conjugated mouse Syk antibody (Cat. No. 552476) or a FITC conjugated mouse isotype control. Cells were washed and analyzed by flow cytometry.
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      BD Pharmingen™
      Human (QC Testing)
      Mouse IgG2a, κ
      Human Syk peptide aa. 314-339
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      AB_394399
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation and Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      推奨アッセイ手順

      Additional applications: The purified format of clone 4D10 is useful in Western blot. Cat. No. 551816 or 551817 are recommended for this application.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      552476 Rev. 3
      抗体の詳細
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      4D10

      Syk is a member of the family of non-receptor type protein-tyrosine kinases and plays a crucial role in lymphocyte signaling and development. Syk is expressed in all haematopoietic cells and contributes to the signal transduction process by binding to a tyrosine-based activation motif (ITAM) of immune receptors, including Igα, TCRζ, CD3ε, FcεRIβ, and FceRIγ. Upon receptor activation, Syk binds to phosphorylated ITAMs via its two N-terminal SH2 domains thereby activating Syk and causing tyrosines in Syk to undergo auto-phosphorylation or phosphorylation. These phosphorylated sites can act as binding sites for other signaling molecules or help to regulate enzymatic activity. For example, in mast cells, Syk can activate downstream targets such as phospholipase Cγ1 and VAV. Studies in Syk-/- mast cells identified defects in both the ERK-MAP and JNK-MAP kinase pathways. Syk migrates as 72 kDa in SDS/PAGE. (Predicted MW from SWISS-PROT is 72 kDa; Accession number P43405).

      552476 Rev. 3
      フォーマットの詳細
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      552476 Rev.3
      引用＆参考文献
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      Development References (4)

      1. Chu DH, van Oers NS, Malissen M, Harris J, Elder M, Weiss A. Pre-T cell receptor signals are responsible for the down-regulation of Syk protein tyrosine kinase expression. J Immunol. 1999; 163(5):2610-2620. (Immunogen: Flow cytometry, Western blot). View Reference
      2. Costello PS, Turner M, Walters AE, et al. Critical role for the tyrosine kinase Syk in signalling through the high affinity IgE receptor of mast cells. Oncogene. 1996; 13(12):2595-2605. (Biology). View Reference
      3. Latour S, Veillette A. Proximal protein tyrosine kinases in immunoreceptor signaling. Curr Opin Immunol. 2001; 13:299-306. (Biology).
      4. Turner M, Schweighoffer E, Colucci F, Di Santo JP, Tybulewicz VL. Tyrosine kinase SYK; essential functions for immunoreceptor signaling. Immunol Today. 2000; 21:148-154. (Biology). View Reference
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      552476 Rev. 3

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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