Purified Mouse Anti-Intersectin/ESE-1

BD Transduction Laboratories™ Purified Mouse Anti-Intersectin/ESE-1

Name: Purified Mouse Anti-Intersectin/ESE-1
Brand: Purified Mouse Anti-Intersectin/ESE-1
SKU: 611574

クローン 29/Intersectin

(RUO)

Western blot analysis of Intersectin on rat cerebrum lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-Intersectin antibody.

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製品詳細

Brand: BD Transduction Laboratories™

Reactivity: Rat (QC Testing), Mouse,Chicken (Tested in Development)

Isotype: Mouse IgG1

Immunogen: Mouse Ese-1 aa. 800-909

Application: Western blot (Routinely Tested), Immunofluorescence (Not Recommended)

Target Molecular Weight: 140/200 kDa

Concentration: 250 µg/ml

RRID: AB_399020

Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

推奨アッセイ手順

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

611574 Rev. 1

抗体の詳細

29/Intersectin

ESE-1 (epithelium-specific, EH domain and SH3 domain regulator of endocytosis) contains two N-terminal EH (Ep15 homology) domains, a central coiled-coil domain, and five C-terminal SH3 domains. ESE-1 is also known as the adaptor protein Intersectin. Intersectin constitutively associates with Eps15 to form a complex that is analogous to the yeast Pan1p-End3p complex, which is required for endocytosis and organization of the actin cytoskeleton. The EH, coiled-coil, and SH3 domains of intersectin bind epsin family proteins, SNAP25 and SNAP23, and dynamin, respectively. Thus, intersectin functions in complex with Eps15 to regulate endocytosis by linking components of the endocytic machinery.

611574 Rev. 1

フォーマットの詳細

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

フォーマット: Purified

611574 Rev.1

引用＆参考文献

View product citations for antibody "611574" on CiteAb

Development References (2)

Okamoto M, Schoch S, Sudhof TC. EHSH1/intersectin, a protein that contains EH and SH3 domains and binds to dynamin and SNAP-25. A protein connection between exocytosis and endocytosis. J Biol Chem. 1999; 274(26):18446-18454. (Biology). View Reference
Sengar AS, Wang W, Bishay J, Cohen S, Egan SE. The EH and SH3 domain Ese proteins regulate endocytosis by linking to dynamin and Eps15. EMBO J. 1999; 18(5):1159-1171. (Biology). View Reference

611574 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.