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Purified Mouse Anti-Human Maspin
Purified Mouse Anti-Human Maspin
Western blot analysis of maspin in A- 431 human epidermal carcinoma cell lysates. The G167-70 antibody was titrated between 1 µg/ml to 0.04 µg/ml (lanes 1-3) and identifies maspin at ~ 42 kD.
Western blot analysis of maspin in A- 431 human epidermal carcinoma cell lysates. The G167-70 antibody was titrated between 1 µg/ml to 0.04 µg/ml (lanes 1-3) and identifies maspin at ~ 42 kD.
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BD Pharmingen™
Human (QC Testing)
Mouse IgG2b
Recombinant Human Maspin
Western blot (Routinely Tested)
42 kDa
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.


Applications include western blot analysis (1-2 µg/ml). A-431 human epidermal carcinoma cells (ATCC CRL 1555) are recommended as a positive control for this application.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
554292 Rev. 7
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Maspin is a 42 kDa protein that may play a role in human breast cancer by functioning as a tumor suppressor.  Maspin is expressed in normal mammary epithelial cells, but there is a loss of expression in invasive breast cancer including lymph node and distant metastases. Further, maspin is not expressed in most mammary carcinoma cell lines. Mammary carcinoma cells transfected with recombinant maspin have a reduced ability to migrate through reconstituted basement membrane and lose their ability to form tumors in nude mice, providing support for maspin as a tumor suppressor protein. Maspin has homology to members of the serpin family of protease inhibitors, some of which have been implicated in the regulation of invasion and metastasis. Yet protease inhibitory activity has not been detected using recombinant maspin.  It has been suggested that maspin, unlike most serpins, may be a ligand-binding serpin rather than a protease inhibitor. However, the question of whether or not maspin has protease inhibitor activity remains to be determined. G167-70 is specific for human maspin. It detects maspin as a 42 kD protein in western blots of normal human mammary epithelial cells. Many mammary tumor cell lines including MDA-MB-231 (ATCC HTB 26) and MDA-MB-435S (ATCC HTB 129) lack maspin expression and are suitable as negative controls. Purified recombinant human maspin from a yeast expression system was used as immunogen.

554292 Rev. 7
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
554292 Rev.7
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View product citations for antibody "554292" on CiteAb

Development References (3)

  1. Hopkins PC, Whisstock J. Function of maspin. Science. 1994; 265(5180):1893-1894. (Biology). View Reference
  2. Sheng S, Pemberton PA, Sager R. Production, purification, and characterization of recombinant maspin proteins. J Biol Chem. 1994; 269(49):30988-30993. (Immunogen). View Reference
  3. Zou Z, Anisowicz A, Hendrix MJ, et al. Maspin, a serpin with tumor-suppressing activity in human mammary epithelial cells. Science. 1994; 263(5146):526-529. (Biology). View Reference
554292 Rev. 7

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.