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Serum Rabbit Anti-Cyclin C

Serum Rabbit Anti-Cyclin C

クローン Polyclonal

(RUO)
Serum Rabbit Anti-Cyclin C
Western blot analysis of cyclin C in NIH 3T3 mouse fibroblasts. The polyclonal antibodies (Cat. No. 558903) was titrated from 1:4000 to 1:12,000 (lanes 1 to 3) and identifies cyclin at ~ 36 kD. The identity of the higher molecular weight band (~ 45 kD) has not been determined, but may represent an alternative splice product of the cyclin C gene.
Western blot analysis of cyclin C in NIH 3T3 mouse fibroblasts. The polyclonal antibodies (Cat. No. 558903) was titrated from 1:4000 to 1:12,000 (lanes 1 to 3) and identifies cyclin at ~ 36 kD. The identity of the higher molecular weight band (~ 45 kD) has not been determined, but may represent an alternative splice product of the cyclin C gene.
製品詳細
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BD Pharmingen™
Mouse (QC Testing), Human (Tested in Development)
Human Cyclin C polypeptide fragment aa. 290-303
Western blot (Routinely Tested), Immunoprecipitation (Tested During Development)
36 kDa
AB_397154
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


推奨アッセイ手順

The antibodies may be used for western blot analysis (1:1000) and immunoprecipitation (1:100). Cyclin C has a molecular weight of 36 kD; however a 45 kD protein may also be observed in some cell types using the antiserum. The identity of this protein has not been determined, but may represent an alternative splice product of the cyclin C gene, as has been identified in other species. HeLa human cervical carcinoma (ATCC CCL 2) and NIH 3T3 mouse fibroblasts (ATCC CRL 1658) are suggested as a positive controls for these applications.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
558903 Rev. 3
抗体の詳細
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Poly1154

Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins contain a conserved amino acid sequence motif, the cyclin box, which allows their binding to cdks to form active complexes that regulate progression of the cell cycle. More recently, cyclins are being shown to have additional functions not restricted to cell cycle regulation. Thus, cyclins have been placed into functional groups as follows: Group 1 cyclins (A, B, D1, D2, D3, E and F) function primarily in cell cycle reguation; Group 2 cyclins (C and H) also play a role in transcriptional regulation; Group 3 cyclins (G1, G2 and I) may play a role distinct from either cell cycle or transcriptional regulation. Cyclin C has been identified in both human and chicken species. Like many cyclins, the expression of cyclin C oscillates throughout the cell cycle, with a peak observed during G1 phase. Cyclin C may play a dual role within the cell in its ability to regulate both cell cycle progression as well as gene transcription. Cyclin C associates with Cdk8, forming a complex which can induce gene transcription of Cdc2 (Cdk1). Because Cdc2 kinase is important for cell entry into mitosis, cyclin C's ability to regulate cell cycle progression may be attributed, in part, to modulation of Cdc2 protein expression.6 Cyclin C has a predicted molecular weight of 36 kD. The polyclonal antiserum reacts with human and mouse cyclin C. A polypeptide fragment containing amino acids 290-303 (QGPNGSQNSSYSQS) at the C-terminus of human cyclin C, with the addition of a C-terminal cysteine (C) residue to facilitate conjugation to KLH, was used as immunogen.

558903 Rev. 3
引用&参考文献
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Development References (6)

  1. Bates S, Rowan S, Vousden KH. Characterisation of human cyclin G1 and G2: DNA damage inducible genes. Oncogene. 1996; 13(5):1103-1109. (Biology). View Reference
  2. Demetrick DJ, Matsumoto S, Hannon GJ, et al. Chromosomal mapping of the genes for the human cell cycle proteins cyclin C (CCNC), cyclin E (CCNE), p21 (CDKN1) and KAP (CDKN3). Cytogenet Cell Genet. 1995; 69(3-4):190-192. (Biology). View Reference
  3. Li H, Lahti JM, Kidd VJ. Alternatively spliced cyclin C mRNA is widely expressed, cell cycle regulated, and encodes a truncated cyclin box. Oncogene. 1996; 13(4):705-712. (Biology). View Reference
  4. Liu ZJ, Ueda T, Miyazaki T, et al. A critical role for cyclin C in promotion of the hematopoietic cell cycle by cooperation with c-Myc. Mol Cell Biol. 1998; 18(6):3445-3454. (Biology). View Reference
  5. Sherr CJ. Mammalian G1 cyclins. Cell. 1993; 73(6):1059-1065. (Biology). View Reference
  6. Tassan JP, Jaquenoud M, Léopold P, Schultz SJ, Nigg EA. Identification of human cyclin-dependent kinase 8, a putative protein kinase partner for cyclin C. Proc Natl Acad Sci U S A. 1995; 92(19):8871-8875. (Biology). View Reference
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558903 Rev. 3

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