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Purified Mouse Anti-Human CD247 with Control

Purified Mouse Anti-Human CD247 with Control

クローン 8D3

(RUO)
Purified Mouse Anti-Human CD247 with Control
Western blot analysis of CD3ζ. Lysate from Jurkat cells was probed with anti-CD3ζ (clone 8D3, Comp. No. 51-6527GR) at concentrations of 0.5 (lane 1), 0.25 (lane 2), and 0.125 µg/ml (lane 3). CD3ζ is identified as a band of 16 kDa.
Western blot analysis of CD3ζ. Lysate from Jurkat cells was probed with anti-CD3ζ (clone 8D3, Comp. No. 51-6527GR) at concentrations of 0.5 (lane 1), 0.25 (lane 2), and 0.125 µg/ml (lane 3). CD3ζ is identified as a band of 16 kDa.
製品詳細
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BD Pharmingen™
Human (QC Testing), Mouse (Tested in Development)
Western blot (Routinely Tested), Immunoprecipitation (Reported)
RUO
AB_394011


説明

The T-cell receptor (TCR), expressed by thymus-derived lymphocytes, is a multi-component complex responsible for recognizing antigen in the context of MHC molecules. Ti is the antigen-specific binding component of the TCR. On the majority of peripheral T lymphocytes Ti is present as a disulfide-linked α-β heterodimer. A minor fraction express Ti as a γ-δ heterodimer. The Ti subunits are Ig-like, each containing V and C regions. Ti is noncovalently associated with an invariant set of molecules referred to as the CD3 subunits. CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes. In the mouse, it consists of five chains, γ (gamma), δ (delta), ε (epsilon), ζ (zeta), and p21. The relative masses of the mouse CD3 chains are 21, 28, 26, 32 (dimer), and 21 kDa respectively.

The 8D3 antibody recognizes the ζ chain of the human and mouse T cell antigen receptor-associated CD3 complex. It reacts with 16 kDa unphosphorylated monomers and 32 kDa unphosphorylated dimers. It does not appear to recognize 21 kDa phosphorylated monomers or 42 kDa phosphorylated dimers. A synthetic peptide corresponding to a region of human CD3ζ was used as immunogen.

推奨アッセイ手順

Jurkat control lysate [50 µg (1 µg/µl) is provided as a Western blot positive control (Comp. 51-16526N; store lysate at -20°C). Additional control lysate (Cat. No. 611451) is sold separately as a ready-to-use western blot control.

製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
551034 Rev. 3
構成品
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説明 数量/容量 Part Number EntrezGene ID
Purified Mouse Anti-Human CD247 50 µg (3 ea) 51-6527GR N/A
Jurkat Cell Lysate 50 µg (1 ea) 51-16526N N/A
551034 Rev. 3
引用&参考文献
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開発者向け参考資料 (1)

  1. Hussey RE, Clayton LK, Diener A, et al. Overexpression of CD3 eta during thymic development does not alter the negative selection process. J Immunol. 1993; 150(4):1183-1194. (Biology). 参考文献を見る
551034 Rev. 3

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