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BD® Flex Single-Cell Multiplexing Kit C, Flex Sample Tag 13-18
(RUO)BD Genomics Rhapsody instrument and Precise reagent kit.
BD® Flex Single-Cell Multiplexing Kit C, Flex Sample Tag 13-18
Regulatory Statusの凡例
Becton, Dickinson and Companyの書面による明示的な許諾を得た使用以外での製品の使用は固く禁じられています。
説明
The BD® Flex Sample Multiplexing Kit C Tag 13-18 contains the necessary reagents to support flexible labelling up to 6 single-cell samples (Flex SMK Sample Tags 13-18). If combined with Flex SMK A, B, and D Kits, up to 24 samples can be labelled and pooled before single-cell capture with the BD Rhapsody™ Single-Cell Analysis system and BD Rhapsody™ Express Single-Cell Analysis system.
The E31-1459 monoclonal antibody specifically recognizes R-Phycoerythrin (PE), a commonly used fluorochrome for flow cytometry. The E31-1459 hybridoma was generated using purified R-Phycoerythrin as the immunogen. The E31-1459 antibody allows for the use of any R-PE conjugated primary antibody to stain sample cells of interest with a two-step staining approach, regardless of cell type or species origin. The binding of the E31-1459 antibody to PE does not quench the fluorescence of the PE molecule. E31-1459 antibody also binds to R-PE tandem fluorophores (eg, PE-Cy™7). User should be mindful if using a multi-color flow sorting panel with PE or PE-tandem conjugates prior to single cell multiomic analysis.
調製と保管
推奨アッセイ手順
Please refer to protocols Single Cell Labeling with the BD® Flex Single-Cell Multiplexing Kits (Doc ID: 23-24311) and Single Cell Labeling with the BD® Flex Single-Cell Multiplexing Kits and BD® AbSeq Ab-Oligos (Doc ID:23-24312). Before using reagents, see the BD Rhapsody™ Single-Cell Analysis System Instrument User Guide (Doc ID: 214062) or the BD Rhapsody™ Express Single-Cell Analysis System Instrument User Guide (Doc ID: 214063).
Use standard laboratory safety protocols. Read and understand the safety data sheets (SDSs) before handling chemicals. To obtain SDSs, go to regdocs.bd.com or contact BD Biosciences technical support at scomix@bdscomix.bd.com.
Warning: All biological specimens and materials coming in contact with them are considered biohazardous. Handle as if capable of transmitting infection and dispose of with proper precautions in accordance with federal, state, and local regulations. Never pipette by mouth. Wear suitable protective clothing, eyewear, and gloves.
製品通知
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For U.S. patents that may apply, see bd.com/patents.
コンパニオン製品
説明 | 数量/容量 | Part Number | EntrezGene ID |
---|---|---|---|
Flex Single-Cell Multiplexing Kit C Sample Tag 13 | 2 Tests (1 ea) | 51-9021569 | N/A |
Flex Single-Cell Multiplexing Kit C Sample Tag 14 | 2 Tests (1 ea) | 51-9021570 | N/A |
Flex Single-Cell Multiplexing Kit C Sample Tag 15 | 2 Tests (1 ea) | 51-9021571 | N/A |
Flex Single-Cell Multiplexing Kit C Sample Tag 16 | 2 Tests (1 ea) | 51-9021572 | N/A |
Flex Single-Cell Multiplexing Kit C Sample Tag 17 | 2 Tests (1 ea) | 51-9021573 | N/A |
Flex Single-Cell Multiplexing Kit C Sample Tag 18 | 2 Tests (1 ea) | 51-9021574 | N/A |
開発者向け参考資料 (3)
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Erickson JR, Mair F, Bugos G, et al. AbSeq Protocol Using the Nano-Well Cartridge-Based Rhapsody Platform to Generate Protein and Transcript Expression Data on the Single-Cell Level.. STAR Protoc. 2020; 1(2):100092. (Methodology). 参考文献を見る
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Mavrangelos C, Swart B, Nobbs S, Nicholson IC, Macardle PJ, Zola H. Detection of low-abundance membrane markers by immunofluorescence--a comparison of alternative high-sensitivity methods and reagents.. J Immunol Methods. 2004; 289(1-2):169-78. (Clone-specific: Flow cytometry). 参考文献を見る
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Shum EY, Walczak EM, Chang C, Christina Fan H. Quantitation of mRNA Transcripts and Proteins Using the BD Rhapsody™ Single-Cell Analysis System.. Adv Exp Med Biol. 2019; 1129:63-79. (Methodology). 参考文献を見る
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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