Preparation and Storage
推奨アッセイ手順
Applications include immunoprecipitation (1-2 µg/1x106 cells), western blot analysis (1-2 µg/ml) and immunohistochemistry of paraformaldehyde-fixed cultured cells (0.5-2.5 µg/ml) and acetone-fixed, frozen and formalin-fixed paraffin-embedded (10 µg/ml) tissue sections. MCF7 human breast carcinoma cells (ATCC HTB-22) are suggested as a positive control. WI-38 human lung fibroblasts (ATCC CCL-75) treated with doxorubin (Adriamycin) can also be used as a positive control.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
p21 belongs to a class of tumor suppressors including p16 and p27 which control progression through the cell cycle by inhibiting the activity of cyclin-cdk complexes. p21 is also known as senescent cell-derived inhibitor 1 (Sdi1), wild-type p53-activated fragment 1 (Waf1), Cdk-interacting protein 1 (Cip1), p21 and p53-regulated inhibitor of Cdks (Pic1). p21 mRNA is expressed at higher levels in senescent fibroblasts than in actively growing cells. When introduced into proliferating foreskin fibroblasts, p21 inhibits DNA synthesis and cell cycle arrest. p53 can activate p21 gene expression via a p53 binding site identified in the promoter of the p21 gene.
Development References (8)
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Clarke AS, Lotz MM, Chao C, Mercurio AM. Activation of the p21 pathway of growth arrest and apoptosis by the beta 4 integrin cytoplasmic domain. J Biol Chem. 1995; 270(39):22673-22676. (Clone-specific: Immunohistochemistry). View Reference
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Halevy O, Novitch BG, Spicer DB, et al. Correlation of terminal cell cycle arrest of skeletal muscle with induction of p21 by MyoD. Science. 1995; 267(5200):1018-1021. (Clone-specific: Immunohistochemistry, Immunoprecipitation). View Reference
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Hunter T. Braking the cycle. Cell. 1993; 75(5):839-841. (Biology). View Reference
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Noda A, Ning Y, Venable SF, Pereira-Smith OM, Smith JR. Cloning of senescent cell-derived inhibitors of DNA synthesis using an expression screen. Exp Cell Res. 1994; 211(1):90-98. (Biology). View Reference
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Xiong Y, Hannon GJ, Zhang H, Casso D, Kobayashi R, Beach D. p21 is a universal inhibitor of cyclin kinases. Nature. 1993; 366(6456):701-704. (Biology). View Reference
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Zhang W, Grasso L, McClain CD. p53-independent induction of WAF1/CIP1 in human leukemia cells is correlated with growth arrest accompanying monocyte/macrophage differentiation. Cancer Res. 1995; 55(3):668-674. (Clone-specific: Western blot). View Reference
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el-Deiry WS, Harper JW, O'Connor PM, et al. WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis. Cancer Res. 1994; 54(5):1169-1174. (Biology). View Reference
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el-Deiry WS, Tokino T, Velculescu VE, et al. WAF1, a potential mediator of p53 tumor suppression. Cell. 1993; 75(4):817-825. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
