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Purified Mouse Anti-TLS
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BD Transduction Laboratories™
Human (QC Testing), Rat (Tested in Development)
Mouse IgG1
Human TLS aa. 2-117.
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
65 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611385 Rev. 1
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Chromosomal translocations in leukemias and malignancies of non-hematopoietic tissues usually result in the fusion of genes and the production of novel fusion proteins. Translocated in liposarcoma (TLS/FUS) was identified through its fusion to the transcription factor CHOP in human myxoid liposarcoma. In addition, the fusion of TLS to the transcription factor ERG is involved in human acute myeloid leukemia. TLS contains an N-terminal Gln-, Ser-, and Tyr-rich region (QSY) that is thought to be a potent transactivator when fused with transcription factors. In its C-terminus, TLS contains a ribonucleoprotein consensus sequence (RNP-CS) and Arg-Gly-Gly (RGG) repeats that have been implicated in RNA binding. This feature allows it to function in shuttling of RNA from the nucleus. TLS also interacts with Ser-Arg proteins that regulate RNA splicing. Interestingly, TLS also interacts with the DNA binding domains of the estrogen, thyroid hormone, and glucocorticoid receptors. Thus, TLS is a multifunctional protein that is involved in RNA transport, nuclear receptor function, and gene transactivation when fused with transcription factors.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611385 Rev. 1
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
611385 Rev.1
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Development References (5)

  1. Hallier M, Lerga A, Barnache S, Tavitian A, Moreau-Gachelin F. The transcription factor Spi-1/PU.1 interacts with the potential splicing factor TLS. J Biol Chem. 1998; 273(9):4838-4842. (Biology). View Reference
  2. Morohoshi F, Ootsuka Y, Arai K, et al. Genomic structure of the human RBP56/hTAFII68 and FUS/TLS genes. Gene. 1998; 221(2):191-198. (Biology). View Reference
  3. Powers CA, Mathur M, Raaka BM, Ron D, Samuels HH. TLS (translocated-in-liposarcoma) is a high-affinity interactor for steroid, thyroid hormone, and retinoid receptors. Mol Endocrinol. 1998; 12(1):4-18. (Biology). View Reference
  4. Yang L, Embree LJ, Tsai S, Hickstein DD. Oncoprotein TLS interacts with serine-arginine proteins involved in RNA splicing. J Biol Chem. 1998; 273(43):27761-27764. (Biology). View Reference
  5. Zinszner H, Sok J, Immanuel D, Yin Y, Ron D. TLS (FUS) binds RNA in vivo and engages in nucleo-cytoplasmic shuttling. J Cell Sci. 1997; 110(15):1741-1750. (Biology). View Reference
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611385 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.