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Purified Mouse Anti-hRAD9
製品詳細
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Human hRAD9 aa. 264-370
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
60 kDa
250 µg/ml
AB_398850
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611324 Rev. 1
抗体の詳細
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56/hRad9

Cell cycle checkpoints are regulatory mechanisms that prevent cell cycle progression in the presence of DNA damage or incompletely replicated DNA.  Many of the genes required for cell-cycle arrest are also involved in DNA repair, apoptosis, and induction of transcription.  In yeast and humans, hRAD9 plays a role in cell cycle arrest during the G2 phase before entry into mitosis.  Phosphorylated hRAD9 is found in the nucleus after DNA damage, and forms DNA damage-responsive complexes with other putative checkpoint control proteins, such as hRAD1 and hHUS1.  Expression of hRAD9 in S. pombe rad9::ura4+ cells restores resistance to the DNA synthesis inhibitor hydroxyurea and gamma rays.  In addition, hRAD9 binds the anti-apoptotic proteins, Bcl-2 and Bcl-xL , and antisense hRAD9 RNA suppresses DNA-damage induced cell death.  Thus, hRAD9 may be an important component of protein complexes that regulate cell cycle progression, as well as apoptosis, in response to DNA damage.

611324 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611324 Rev.1
引用&参考文献
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開発者向け参考資料 (5)

  1. Komatsu K, Miyashita T, Hang H. Human homologue of S. pombe Rad9 interacts with BCL-2/BCL-xL and promotes apoptosis. Nat Cell Biol. 2000; 2(1):1-6. (Biology). 参考文献を見る
  2. Lieberman HB, Hopkins KM, Nass M, Demetrick D, Davey S. A human homolog of the Schizosaccharomyces pombe rad9+ checkpoint control gene. Proc Natl Acad Sci U S A. 1996; 93(24):13890-13895. (Biology). 参考文献を見る
  3. St Onge RP, Udell CM, Casselman R, Davey S. The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1. Mol Cell Biol. 1999; 10(6):1985-1995. (Biology). 参考文献を見る
  4. Volkmer E, Karnitz LM. Human homologs of Schizosaccharomyces pombe rad1, hus1, and rad9 form a DNA damage-responsive protein complex. J Biol Chem. 1999; 274(2):567-570. (Biology). 参考文献を見る
  5. Yoshida K, Komatsu K, Wang HG, Kufe D. c-Abl tyrosine kinase regulates the human Rad9 checkpoint protein in response to DNA damage. Mol Cell Biol. 2002; 22(10):3292-32300. (Biology: Immunoprecipitation, Western blot). 参考文献を見る
すべて表示する (5) 表示項目を減らす
611324 Rev. 1

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