Immunofluorescence staining of SKN cells (Human neuroblastoma).
Immunofluorescence staining of SKN cells (Human neuroblastoma).
Preparation and Storage
推奨アッセイ手順
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
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The family of 14-3-3 proteins has been implicated in numerous cellular processes such as neurotransmission, phospholipase activity, and calcium signaling. The 14-3-3 protein can associate with several proteins involved in cell proliferation, such as Raf-1, Bcr-Abl, middle T antigen, and CDC25 phosphatase. It appears that 14-3-3 proteins enhance the kinase activity of the Raf proto-oncogene equally as well as Ras, thus, implicating 14-3-3 proteins in the Raf/Ras/Map kinase signaling pathway. However, in spite of 14-3-3 direct association with the CDC25 phosphatase, this interaction does not increase the phosphatase activity. It has been reported that this antibody detects 14-3-3 subunits β, γ, η, θ, and ζ , but not ε nor σ.
Development References (5)
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Ayllon V, Cayla X, Garcia A, et al . Bcl-2 targets protein phosphatase 1 alpha to Bad. J Immunol. 2001; 166(12):7345-7352. (Biology: Immunoprecipitation, Western blot). View Reference
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Conklin DS, Galaktionov K, Beach D.. 14-3-3 proteins associate with cdc25 phosphatases.. Proc Natl Acad Sci U S A. 1995; 92(17):7892-7896. (Biology). View Reference
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Freed E, Symons M, Macdonald SG, McCormick F, Ruggieri R.. Binding of 14-3-3 proteins to the protein kinase Raf and effects on its activation.. Science. 1994 ; 265(5179):1713-1716. (Biology: Western blot). View Reference
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Mamane Y, Grandvaux N, Hernandez E, et al. Repression of IRF-4 target genes in human T cell leukemia virus-1 infection. Nature. 2002; 21(44):6751-6765. (Biology: Immunofluorescence, Immunohistochemistry, Western blot). View Reference
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Ostrerova N, Petrucelli L, Farrer M, Mehta N,. alpha-Synuclein shares physical and functional homology with 14-3-3 proteins.. J Neurosci. 1999; 19(14):5782-5791. (Biology: Immunoprecipitation, Western blot). View Reference
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