Multicolor flow cytometric analysis of Vβ 13 TCR expressed on mouse lymph node T cells. C57BL/6 mouse lymph node cells were stained with PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049) and PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033) and either V450 Mouse IgG1, κ Isotype Control (Cat. No. 560373, left panel) or V450 Mouse Anti-Mouse Vβ 13 TCR (Cat. No. 561539, right panel). Two-color flow cytometric dot plots showing the expression of Vβ 13 TCR (or Ig Isotype Control staining) versus CD4 and CD8 were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD™ LSR II.
Multicolor flow cytometric analysis of Vβ 13 TCR expressed on mouse lymph node T cells. C57BL/6 mouse lymph node cells were stained with PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049) and PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033) and either V450 Mouse IgG1, κ Isotype Control (Cat. No. 560373, left panel) or V450 Mouse Anti-Mouse Vβ 13 TCR (Cat. No. 561539, right panel). Two-color flow cytometric dot plots showing the expression of Vβ 13 TCR (or Ig Isotype Control staining) versus CD4 and CD8 were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD™ LSR II.
製品詳細
BD Horizon™
TCR V beta 13; TCR Vβ 13; TCR Vβ13; Vβ 13 T-cell Receptor
Mouse (QC Testing)
Mouse C57BR IgG1, κ
Not reported
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_10924602
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ V450 under optimum conditions, and unreacted BD Horizon™ V450 was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
Stain Buffer (FBS) RUO
サイズ
500 mL
カタログ番号
554656
Stain Buffer (BSA) RUO
サイズ
500 mL
カタログ番号
554657
V450 Mouse IgG1, κ Isotype Control RUO
サイズ
0.1 mg
カタログ番号
560373
PE Rat Anti-Mouse CD4 RUO
サイズ
0.1 mg
カタログ番号
553048
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PE Rat Anti-Mouse CD4 RUO
サイズ
0.2 mg
カタログ番号
553049
PE Rat Anti-Mouse CD8a RUO
サイズ
0.1 mg
カタログ番号
553032
561539 Rev. 2
抗体の詳細
MR12-3
The MR12-3 monoclonal antibody specifically binds to the Vβ 13 T-cell Receptor (TCR) of mice having the b haplotype (e.g., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb gene complex. The Tcrb-V13 gene locus is deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) or c (e.g., RIII) haplotype.
The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.
561539 Rev. 2
フォーマットの詳細
V450
BD Horizon™ V450 Dye is part of the BD Horizon™ violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 405-nm and an emission maximum (Em Max) at 450-nm. BD Horizon™ V450, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450-nm (e.g., a 450/50-nm bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
V450
Violet 405 nm
405 nm
450 nm
561539 Rev.2
引用＆参考文献
Development References (3)
-
Behlke MA, Chou HS, Huppi K, Loh DY. Murine T-cell receptor mutants with deletions of beta-chain variable region genes. Proc Natl Acad Sci U S A. 1986; 83(3):767-771. (Biology). View Reference
-
Chen D, Lee F, Cebra JJ, Rubin DH. Predominant T-cell receptor Vbeta usage of intraepithelial lymphocytes during the immune response to enteric reovirus infection.. J Virol. 1997; 71(5):3431-6. (Biology). View Reference
-
Haqqi TM, Banerjee S, Anderson GD, David CS. RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes. J Exp Med. 1989; 169(6):1903-1909. (Biology). View Reference
561539 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
