V450 Hamster anti-Mouse CD3e

BD Horizon™ V450 Hamster anti-Mouse CD3e

Name: V450 Hamster anti-Mouse CD3e
Brand: BD Horizon™
SKU: 560804

クローン 500A2

(RUO)

Flow cytometric analysis of CD3 expression on mouse splenocytes using BD Horizon™ V450 anti-mouse CD3e. BALB/c mouse spleen cells were stained simultaneously with BD Horizon™ V450-conjugated anti-mouse CD3e antibody and Alexa Fluor® 647 anti-mouse CD45R (Cat. No.557683; right panel) or FITC anti-mouse CD4 (Cat. No.553047 ; left panel) antibody. Two-color flow cytometric dot plots showing the correlated expression patterns of CD3e and CD45R or CD3e and CD4 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSRII System.

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製品詳細

Brand: BD Horizon™

Alternative Name: CD3; CD3 epsilon; Cd3e; CD3ε; T3e

Reactivity: Mouse (QC Testing)

Isotype: Syrian Hamster IgG2, κ

Immunogen: Mouse T-cell receptor

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 12501

RRID: AB_2034005

Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ V450 under optimum conditions, and unreacted BD Horizon™ V450 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.

関連製品

V450 Hamster IgG2, κ Isotype Control RUO

サイズ 0.1 mg カタログ番号 560563

Alexa Fluor® 647 Rat Anti-Mouse CD45R RUO

サイズ 0.1 mg カタログ番号 557683

FITC Rat Anti-Mouse CD4 RUO

サイズ 0.5 mg カタログ番号 553047

560804 Rev. 1

抗体の詳細

500A2

The 500A2 monoclonal antibody specifically binds to the 25-kDa ε chain of the T-cell receptor-associated CD3 complex expressed on mouse thymocytes, mature T lymphocytes, and NKT cells. Plate-bound and soluble forms of the 500A2 antibody can activate T cells in vitro. Activation of a mouse T-cell clone by the 500A2 antibody can be blocked by Fab fragments of the GK1.5 anti-CD4 antibody. This suggests that the 500A2 antibody may bind an epitope on CD3e close to a site at which CD4 associates with the T-cell receptor. The 500A2 antibody reportedly does not to crossreact with rat leukocytes.

The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

560804 Rev. 1

フォーマットの詳細

V450

BD Horizon™ V450 Dye is part of the BD Horizon™ violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 405-nm and an emission maximum (Em Max) at 450-nm. BD Horizon™ V450, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450-nm (e.g., a 450/50-nm bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: V450

励起源: Violet 405 nm

最大励起波長: 405 nm

最大蛍光波長: 450 nm

560804 Rev.1

引用＆参考文献

View product citations for antibody "560804" on CiteAb

Development References (5)

Allison JP, Havran WL, Poenie M, et al. Expression and function of CD3 on murine thymocytes. In: Kappler J, Davis M, ed. The T-Cell Receptor, UCLA Symposia, 73rd Edition. Los Angeles: 1988:33-45.
Havran WL, Poenie M, Kimura J, Tsien R, Weiss A, Allison JP. Expression and function of the CD3-antigen receptor on murine CD4+8+ thymocytes. Nature. 1987; 330(6144):170-173. (Clone-specific). View Reference
Kubo RT, Born W, Kappler JW, Marrack P, Pigeon M. Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors. J Immunol. 1989; 142(8):2736-2742. (Methodology). View Reference
Ortaldo JR, Winkler-Pickett R, Mason AT, Mason LH. The Ly-49 family: regulation of cytotoxicity and cytokine production in murine CD3+ cells. J Immunol. 1998; 160(1):1158-1165. (Clone-specific). View Reference
Portoles P, Rojo J, Golby A, et al . Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation. J Immunol. 1989; 142(12):4169-4175. (Clone-specific). View Reference

すべて表示する (5)

560804 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.