Preparation and Storage
推奨アッセイ手順
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- CF™ is a trademark of Biotium, Inc.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
関連製品
The 1D12 monoclonal antibody specifically binds to CD365, the T-cell immunoglobulin mucin receptor 1 (TIM-1). TIM-1 is expressed on kidney epithelial cells, T cells, and some hematopoietic and non-hematopoietic cells. CD365 (TIM-1) is a type 1 transmembrane glycoprotein that serves as a receptor for hepatitis A virus and is encoded by the HAVCR1 (Hepatitis A virus cellular receptor 1) gene. TIM-1 also serves as a receptor for phosphatidylserine which is exposed on the surface of apoptotic cells. TIM-1 can reportedly mediate the uptake of apoptotic cells through the recognition of phosphatidylserine and thus help maintain tissue homeostasis and self-tolerance. TIM-1 is likewise known as Kidney injury molecule 1 (KIM-1). It is highly expressed by cancerous kidneys, and upregulated in the proximal tubular epithelium and shed into the urine during acute and chronic kidney injury. CD365 (TIM-1) also functions as a costimulatory molecule for immune cells. It is expressed by activated CD4+ T cells and regulates the effector functions (eg, enhanced cytokine production) and survival of differentiated T cells, including those mediating Th2-like immune responses. Other ligands have been described for TIM-1 including TIM-4 and LMIR5 (also known as CD300b) which are expressed by myeloid cells. With respect to disease associations, the HAVCR1 gene has been linked to asthma, allergy, and some autoimmune diseases.
Development References (7)
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Binne LL, Scott ML, Rennert PD. Human TIM-1 associates with the TCR complex and up-regulates T cell activation signals. J Immunol. 2007; 178(7):4342-4350. (Biology). View Reference
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Freeman GJ, Casasnovas JM, Umetsu DT, DeKruyff RH. TIM genes: a family of cell surface phosphatidylserine receptors that regulate innate and adaptive immunity.. Immunol Rev. 2010; 235(1):172-89. (Biology). View Reference
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Kim HY, Eyheramonho MB, Pichavant M, et al. A polymorphism in TIM1 is associated with susceptibility to severe hepatitis A virus infection in humans. J Clin Invest. 2011; 121(3):1111-1118. (Clone-specific: Blocking, Functional assay). View Reference
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Kobayashi N, Karisola P, Pena-Cruz V, et al. TIM-1 and TIM-4 glycoproteins bind phosphatidylserine and mediate uptake of apoptotic cells. Immunity. 2007; 27(6):927-940. (Immunogen: Blocking, Flow cytometry, Functional assay). View Reference
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Manangeeswaran M, Jacques J, Tami C, et al. Binding of hepatitis A virus to its cellular receptor 1 inhibits T-regulatory cell functions in humans. Gastroenterology. 2012; 142(7):1516-1525. (Clone-specific: Blocking, Functional assay). View Reference
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Rennert PD. Novel roles for TIM-1 in immunity and infection. Immunol Lett. 2011; 141(1):28-35. (Clone-specific: Blocking). View Reference
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Rodriguez-Manzanet R, DeKruyff R, Kuchroo VK, Umetsu DT. The costimulatory role of TIM molecules. Immunol Rev. 2009; 229(1):259-270. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
