Skip to main content Skip to navigation
Hamburger Menu
Search icon
Location Selector Location Selector
Japan (Japanese)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Link Arrow
Solutions

Link Arrow
Discover & Learn

Link Arrow
Resources & Tools

Link Arrow
Support

Link Arrow
Close Menu
Website Feedback
Alert Icon
製品詳細
Down Arrow Up Arrow


BD Pharmingen™
Integrin α4; Itga4; Integrin alpha-4; LPAM alpha; VLA-4 alpha; VLA-4a
Mouse (QC Testing)
Rat LEW, also known as Lewis IgG2a, κ
Mouse (C57BL/6xA/J)F[1] Fetal Liver Mast MC/9
Flow cytometry (Routinely Tested), Blocking, Immunohistochemistry-frozen (Reported)
1.0 mg/ml
AB_394776
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
RUO


Preparation and Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
詳細を表示 blue down arrow 表示項目を減らす blue up arrow

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
詳細を表示 blue down arrow 表示項目を減らす blue up arrow
553313 Rev. 12
抗体の詳細
Down Arrow Up Arrow
9C10(MFR4.B)

The 9C10 (MFR4.B) monoclonal antibody specifically binds to the Integrin α4 chain (CD49d), which is expressed as a heterodimer with either of two β chains, β1 or β7. The α4β1 integrin (VLA-4, CD49d/CD29) is expressed on most peripheral lymphocytes, thymocytes, and monocytes; while the α4β7 integrin (LPAM-1) is expressed on peripheral lymphocytes, but on only a small subset of thymocytes. These integrins mediate a variety of cell-cell and cell-matrix interactions, recognizing the ligands VCAM-1 (CD106) and fibronectin. Integrin α4β7 also preferentially binds to the mucosal vascular addressin, MAdCAM-1. Although the 9C10 (MFR4.B) antibody alone has been reported to have little function-blocking activity, it can augment the inhibitory effects of mAb R1-2 (Cat. No. 553153), resulting in almost complete inhibition of VLA-4 binding to VCAM-1.

553313 Rev. 12
フォーマットの詳細
Down Arrow Up Arrow
NA/LE
NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is =0.01 EU/µg (=0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
NA/LE
553313 Rev.12
引用＆参考文献
Down Arrow Up Arrow
View product citations for antibody "553313" on CiteAb

Development References (2)

  1. Halvorson MJ, Coligan JE. Enhancement of VLA integrin receptor function on thymocytes by cAMP is dependent on the maturation stage of the thymocytes. J Immunol. 1995; 155(10):4567-4574. (Clone-specific: Blocking). View Reference
  2. Kinashi T, Springer TA. Adhesion molecules in hematopoietic cells. Blood Cells. 1994; 20(1):25-44. (Immunogen: Blocking). View Reference
553313 Rev. 12

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.