Purified NA/LE Rat Anti-Human and Viral IL-10

BD Pharmingen™ Purified NA/LE Rat Anti-Human and Viral IL-10

Name: Purified NA/LE Rat Anti-Human and Viral IL-10
Brand: Purified NA/LE Rat Anti-Human and Viral IL-10
SKU: 554495

クローン JES3-9D7 (RUO)

製品詳細

Brand: BD Pharmingen™

Reactivity: Human (QC Testing), Viral (Tested in Development)

Isotype: Rat IgG1

Immunogen: Recombinant Human IL-10

Application: ELISA Capture (Routinely Tested), Intracellular staining (flow cytometry), Neutralization (Tested During Development)

Concentration: 1.0 mg/ml

RRID: AB_395432

Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

関連製品

Purified NA/LE Rat IgG1 κ Isotype Control RUO

サイズ 0.5 mg カタログ番号 554682

Purified Rat Anti-Human and Viral IL-10 RUO

サイズ 0.5 mg カタログ番号 554497

Recombinant Mouse IL-4 RUO

サイズ 10 µg カタログ番号 550067

Biotin Anti-Human and Viral IL-10 RUO

サイズ 0.5 mg カタログ番号 554499

Recombinant Human IL-10 RUO

サイズ 5 µg カタログ番号 554611

554495 Rev. 5

抗体の詳細

JES3-9D7

The JES3-9D7 monoclonal antibody specifically reacts with human IL-10 (Interleukin-10) and viral IL-10. The immunogen used to generate the JES3-9D7 hybridoma was recombinant human IL-10 expressed by COS cells. IL-10 is also known as CSIF (Cytokine synthesis inhibitory factor) and (TGIF) T-cell growth inhibitory factor. IL-10 is expressed by various cell types including activated monocytes, macrophages, dendritic cells, mast cells, granulocytes, and lymphocytes. IL-10 is a pleiotropic cytokine that can downregulate proinflammatory immune responses, such as Th1-like responses, while promoting other responses including B cell proliferation and antibody production.

554495 Rev. 5

フォーマットの詳細

NA/LE

NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.

フォーマット: NA/LE

554495 Rev.5

引用＆参考文献

View product citations for antibody "554495" on CiteAb

Development References (4)

Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Biology). View Reference
Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Biology). View Reference
Burdin N, Peronne C, Banchereau J, Rousset F. Epstein-Barr virus transformation induces B lymphocytes to produce human interleukin 10. J Exp Med. 1993; 177(2):295-304. (Biology). View Reference
Gotlieb WH, Abrams JS, Watson JM, Velu TJ, Berek JS, Martinez-Maza O. Presence of interleukin 10 (IL-10) in the ascites of patients with ovarian and other intra-abdominal cancers. Cytokine. 1992; 4(5):385-390. (Biology). View Reference

すべて表示する (4)

554495 Rev. 5

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.