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BD Pharmingen™ Purified NA/LE Mouse Anti-Mouse IL-17F
クローン MM17F8F5 (also known as MM17F8.F5; MM17F.8F5; MM17F-8F5)
(RUO)



Neutralization of mouse IL-17F biological activity with MM17F8F5, a Mouse Anti-Mouse IL-17F monoclonal antibody. In a 96-well microplate, biologically-active recombinant mouse IL-17F protein (0.2 μg/well) was preincubated (1 hr, 37°C) with serial dilutions of Purified NA/LE Mouse Anti-Mouse IL-17F antibody (Cat. No. 563592; μg/ml) or tissue culture medium (as a control). Following the incubation, cells from the NIH-3T3 mouse embryonic cell line were added at 5 x 10^4 cells per well and were cultured at 37°C. After 24 hours of culture, the supernatants from each well were harvested and their concentrations of IL-6 were quantified using a BD™ Cytometric Bead Array Mouse IL-6 Flex Set (Cat. No. 558301). As the MM17F8F5 antibody was serially diluted, a neutralizing Anti-Mouse IL-17F antibody dose-response relationship was observed as shown in the figure.


BD Pharmingen™ Purified NA/LE Mouse Anti-Mouse IL-17F

Regulatory Statusの凡例
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation and Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- CF™ is a trademark of Biotium, Inc.
- Cy is a trademark of GE Healthcare.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品






The MM17F8F5 monoclonal antibody specifically binds to Interleukin-17F (IL-17F) and can neutralize its biological activity. IL-17F is a member of the IL-17 family of cytokines. Among IL-17 family members, IL-17F has the highest amino acid sequence homology to IL-17A. IL-17F is produced by activated CD4+ T helper (Th17) cells, CD8+ T (Tc17) cells and γδ T cells. IL-17F can be secreted as homodimers or as heterodimers with IL-17A. IL-17F and IL-17A have overlapping functions such as inducing epithelial cells and fibroblasts to produce proinflammatory cytokines and chemokines including IL-6, GM-CSF, CXCL1, CCL2, and CCL7. These factors attract and activate neutrophils and other cell types that mediate protective responses against pathogenic microbes or pathologic allergic or autoimmune diseases. IL-17 gene knockout studies have shown that IL-17F and IL-17A have independent functions as well. IL-17F and IL-17A exert their biological function by binding to and signaling through IL-17 receptors comprised of the transmembrane receptor subunits, IL-17RA (CD217) and IL-17RC.
Development References (9)
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Chang SH, Dong C. IL-17F: Regulation, signaling and function in inflammation. Cytokine. 2009; 46(1):7-11. (Biology). View Reference
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Dong C. Th17 cells: Current understanding of their generation and regulation. Eur J Immunol. 2009; 39(3):640-644. (Biology). View Reference
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Hamada H, Garcia-Hernandez MdlL, Reome JB, et al. Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge. J Immunol. 2009; 182(6):3469-3481. (Biology). View Reference
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Ishigame H, Kakuta S, Nagai T, et al. Differential roles of interleukin-17A and -17F in host defense against mucoepithelial bacterial infection and allergic responses. Immunity. 2009; 30(1):108-119. (Biology). View Reference
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Martinez GJ, Zhang Z, Chung Y, et al. Smad3 differentially regulates the induction of regulatory and inflammatory T cell differentiation. J Biol Chem. 2009; 284(51):35283-35286. (Biology). View Reference
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Oda N, Canelos PB, Essayan DM, Plunkett BA, Myers AC, Huang SK. Interleukin-17F induces pulmonary neutrophilia and amplifies antigen-induced allergic response. Am J Respir Crit Care Med. 2005; 171(1):12-13. (Biology). View Reference
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Personal communication. Jacques Van Snick. MM17F8F5 neutralizing antibody. .
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Sutton CE, Lalor SJ, Sweeney CM, Brereton CF, Lavelle EC, Mills KH. Interleukin-1 and IL-23 induce innate IL-17 production from gammadelta T cells, amplifying Th17 responses and autoimmunity. Immunity. 2009; 31(2):331-341. (Biology). View Reference
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Yang XO, Chang SH, Park H, et al. Regulation of inflammatory responses by IL-17F. J Exp Med. 2008; 205(5):1063-1075. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.