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Purified Mouse Anti-Human CD123
製品詳細
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BD Pharmingen™
IL3RA; IL-3RA; IL-3Rα; IL-3R-alpha; Interleukin-3 receptor subunit alpha
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human IL-3Rα Transfected Cell Line
Flow cytometry (Routinely Tested), Immunofluorescence, Western blot (Reported)
0.5 mg/ml
VI C-67
3563
AB_395999
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


推奨アッセイ手順

This antibody is useful for western blotting and for immunofluorescent labelling of cells for fluorescence microscopy or flow cytometry. For flow

cytometric applications, a three-step labelling procedure is recommended for amplifying signal. For example:

1. Incubate (~1 million) cells with ~2 µg of antibody for 45-60 minutes at 4° C. Wash three times in PBS/0.1% sodium azide/1.0% heat -inactivated fetal bovine serum (PBS-FBS).

2. Incubate cells with ~0.5 µg biotinylated goat anti-mouse IgS (Cat. No. 553999) at 4° C for 30 minutes. Wash three times.

3. Incubate cells with ~0.1 µg streptavidin-PE (Cat. No. 554061) at 4° C for 30 minutes. Wash three times. Resuspend in 0.5 ml of PBS-FBS and analyze by flow cytometry.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
555642 Rev. 12
抗体の詳細
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9F5

The 9F5 monoclonal antibody specifically binds to CD123. CD123 is the 70 kDa IL-3 receptor α chain (IL-3Rα) that associates with the 120-140 kDa β subunit (CD131/Common β-chain/βc) to form the functional IL-3 receptor complex. The βc chain is also shared with distinct α chain subunits to form the functional heterodimeric receptors for interleukins IL-5 and GM-CSF. IL-3Rα is expressed on a subset of peripheral blood dendritic cells, myeloid precursors, basophils, mast cells, macrophages, and megakaryocytes. Reports indicate that IL-3Rα is also expressed on lymphocytes. The IL-3R plays an important role in hematopoietic progenitor cell growth and differentiation. This antibody does not block binding of IL-3 to the IL-3 receptor.

555642 Rev. 12
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
555642 Rev.12
引用&参考文献
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Development References (2)

  1. Macardle PJ, Chen Z, Shih CY, et al. Characterization of human leucocytes bearing the IL-3 receptor. Cell Immunol. 1996; 168(1):59-68. (Biology). View Reference
  2. Sun Q, Woodcock JM, Rapoport A, et al. Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist.. Blood. 1996; 87(1):83-92. (Biology). View Reference
555642 Rev. 12

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.