BD Pharmingen™ PerCP-Cy™5.5 Mouse Anti-Pig IFN-γ
クローン P2G10 (RUO)
Multicolor flow cytometric analysis of IFN-γ expression by stimulated pig lymphocytes. Pig peripheral blood mononuclear cells were stimulated for 20 h with Leukocyte Activation Cocktail, with BD GolgiPlug™ (Cat. No. 550583). The cells were fixed using BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were then stained with PE Mouse Anti-Pig CD3 ε antibody (Cat. No. 561485) and PerCP-Cy™5.5 Mouse Anti-Pig IFN-γ antibody (Cat. No. 561481; Left Panel) or a PerCP-Cy™5.5 Mouse IgG1 Isotype Control (Cat No. 550795, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of IFN-γ (or Ig Isotype control staining) versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Multicolor flow cytometric analysis of IFN-γ expression by stimulated pig lymphocytes. Pig peripheral blood mononuclear cells were stimulated for 20 h with Leukocyte Activation Cocktail, with BD GolgiPlug™ (Cat. No. 550583). The cells were fixed using BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were then stained with PE Mouse Anti-Pig CD3 ε antibody (Cat. No. 561485) and PerCP-Cy™5.5 Mouse Anti-Pig IFN-γ antibody (Cat. No. 561481; Left Panel) or a PerCP-Cy™5.5 Mouse IgG1 Isotype Control (Cat No. 550795, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of IFN-γ (or Ig Isotype control staining) versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
製品詳細
BD Pharmingen™
IFNG; IFN-gamma; Interferon gamma; Interferon-gamma
Pig (QC Testing)
Mouse IgG1, κ
Recombinant pig IFN-γ protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_10681057
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence.
- PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis.
- Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- This product is subject to proprietary rights of Amersham Biosciences Corp. and Carnegie Mellon University and made and sold under license from Amersham Biosciences Corp. This product is licensed for sale only for research. It is not licensed for any other use. If you require a commercial license to use this product and do not have one return this material, unopened to BD Biosciences, 10975 Torreyana Rd, San Diego, CA 92121 and any money paid for the material will be refunded.
- This PerCP-conjugated product is sold under license to the following patent: US Patent No. 4,876,190.
- Cy is a trademark of Amersham Biosciences Limited. This conjugated product is sold under license to the following patents: US Patent Nos. 5,486,616; 5,569,587; 5,569,766; 5,627,027.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
Fixation Buffer RUO
サイズ
100 mL
カタログ番号
554655
Perm/Wash Buffer RUO
サイズ
100 mL
カタログ番号
554723
Leukocyte Activation Cocktail, with BD GolgiPlug™ RUO
サイズ
200 µL
カタログ番号
550583
PerCP-Cy™5.5 Mouse IgG1 κ Isotype Control RUO
サイズ
0.1 mg
カタログ番号
550795
PE Mouse Anti-Pig CD3ε RUO
サイズ
50 µg
カタログ番号
561485
561481 Rev. 1
抗体の詳細
P2G10
The P2G10 monoclonal antibody specifically binds to porcine interferon-γ (IFN-γ). The immunogen used to generate the P2G10 hybridoma was recombinant pig IFN-γ protein.
561481 Rev. 1
フォーマットの詳細
PerCP-Cy5.5
PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PerCP-Cy5.5
Blue 488 nm
482 nm
676 nm
561481 Rev.1
引用＆参考文献
View product citations for antibody "561481" on CiteAb
Development References (2)
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Mateu de Antonio E, Husmann RJ, Hansen R, et al. Quantitative detection of porcine interferon-gamma in response to mitogen, superantigen and recall viral antigen. Vet Immunol Immunopathol. 1998; 61(2-4):265-277. (Immunogen: ELISA). View Reference
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Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: IC/FCM Block). View Reference
561481 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.