BD Pharmingen™ PE Rat Anti-Mouse CD83
クローン Michel-19 (RUO)
Analysis of CD83 expression by mature dendritic cells (DC). C57BL/6 bone marrow-derived DC were cultured for 6 days with recombinant mouse GM-CSF (Cat. no. 554586), stimulated for 24 hours with lipopolysaccharide, then stained with FITC Hamster anti-Mouse CD11c mAb HL3 (Cat. no. 557400) and either PE Rat IgG1, κ isotype control mAb R3-34 (Cat. no. 553925, left panel) or PE mAb Michel-19 (right panel). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
Analysis of CD83 expression by mature dendritic cells (DC). C57BL/6 bone marrow-derived DC were cultured for 6 days with recombinant mouse GM-CSF (Cat. no. 554586), stimulated for 24 hours with lipopolysaccharide, then stained with FITC Hamster anti-Mouse CD11c mAb HL3 (Cat. no. 557400) and either PE Rat IgG1, κ isotype control mAb R3-34 (Cat. no. 553925, left panel) or PE mAb Michel-19 (right panel). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
製品詳細
BD Pharmingen™
Mouse (QC Testing)
Rat IgG1, κ
Mouse CD83 Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_647203
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
FITC Hamster Anti-Mouse CD11c RUO
サイズ
0.1 mg
カタログ番号
557400
Recombinant Mouse GM-CSF RUO
サイズ
10 µg
カタログ番号
554586
558205 Rev. 1
抗体の詳細
Michel-19
The Michel-19 antibody reacts with CD83, a member of the immunoglobulin superfamily that is expressed on mature dendritic cells and activated T lymphocytes. Furthermore, thymic cortical epithelial cells express Cd83 transcripts. CD83 is involved in the regulation of T-cell development and immune responses, and its ligand is found on a subpopulation of splenic B lymphocytes.
558205 Rev. 1
フォーマットの詳細
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
558205 Rev.1
引用＆参考文献
View product citations for antibody "558205" on CiteAb
Development References (5)
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Berchtold S, Muhl-Zurbes P, Heufler C, Winklehner P, Schuler G, Steinkasserer A. Cloning, recombinant expression and biochemical characterization of the murine CD83 molecule which is specifically upregulated during dendritic cell maturation. FEBS Lett. 1999; 461:211-216. (Biology). View Reference
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Cramer SO, Trumpfheller C, Mehlhoop U, More S, Fleischer B, von Bonin A. Activation-induced expression of murine CD83 on T cells and identification of a specific CD83 ligand on murine B cells. Int Immunol. 2000; 12(9):1347-1351. (Biology). View Reference
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Fujimoto Y, Tu L, Miller AS, et al. CD83 expression influences CD4+ T cell development in the thymus. Cell. 2002; 108:755-767. (Biology). View Reference
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Wolenski M, Cramer SO, Ehrlich S, Steeg C, Fleischer B, von Bonin A. Enhanced activation of CD83-positive T cells. Scand J Immunol. 2003; 58(3):306-311. (Biology). View Reference
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Wolenski M, Cramer SO, Ehrlich S, et al. Expression of CD83 in the murine immune system. Med Microbiol Immunol (Berl). 2003; 192(4):189-192. (Biology). View Reference
558205 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.