Multi-parameter flow cytometric analysis of CD181 (CXCR1) expression on mouse blood neutrophils (left and middle panel). Mouse blood cells were stained simultaneously with APC Rat Anti-Mouse Ly-6G antibody (Cat. No. 560599), BD Horizon™ BV421 Rat Anti-Mouse CD11b antibody (Cat. No. 562605), and either PE Rat IgG2b, κ Isotype Control (Cat. No. 555848) or PE Rat Anti-Mouse CD181 (CXCR1) antibody (Cat. No. 566383) at 0.5 µg/test. Erythrocytes were then lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The left panel displays two-color flow cytometric plots showing the correlated expression patterns of CD11b versus Ly-6G from gated events with the forward and side light-scatter characteristics of viable cells. The middle panel displays the fluorescence histograms of CD181 (CXCR1) expression (solid line histogram) or Ig Isotype control staining (dashed line histogram) on gated Ly-6G and CD11b double-positive neutrophils. Blood leukocytes that were Ly-6G and CD11b double-negative did not express CD181 (data not shown). Flow cytometric analysis of CD181 (CXCR1) expression on transfected 293F cells (right panel). Untransfected (dashed line histogram) and mouse CD181-transfected (solid line histogram) 293F cells were stained with PE Rat Anti-Mouse CD181 (CXCR1) antibody (Cat.No. 566383) at 0.5 ug/test. The fluorescence histogram showing CD181 (CXCR1) expression was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed on a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
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Multi-parameter flow cytometric analysis of CD181 (CXCR1) expression on mouse blood neutrophils (left and middle panel). Mouse blood cells were stained simultaneously with APC Rat Anti-Mouse Ly-6G antibody (Cat. No. 560599), BD Horizon™ BV421 Rat Anti-Mouse CD11b antibody (Cat. No. 562605), and either PE Rat IgG2b, κ Isotype Control (Cat. No. 555848) or PE Rat Anti-Mouse CD181 (CXCR1) antibody (Cat. No. 566383) at 0.5 µg/test. Erythrocytes were then lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The left panel displays two-color flow cytometric plots showing the correlated expression patterns of CD11b versus Ly-6G from gated events with the forward and side light-scatter characteristics of viable cells. The middle panel displays the fluorescence histograms of CD181 (CXCR1) expression (solid line histogram) or Ig Isotype control staining (dashed line histogram) on gated Ly-6G and CD11b double-positive neutrophils. Blood leukocytes that were Ly-6G and CD11b double-negative did not express CD181 (data not shown). Flow cytometric analysis of CD181 (CXCR1) expression on transfected 293F cells (right panel). Untransfected (dashed line histogram) and mouse CD181-transfected (solid line histogram) 293F cells were stained with PE Rat Anti-Mouse CD181 (CXCR1) antibody (Cat.No. 566383) at 0.5 ug/test. The fluorescence histogram showing CD181 (CXCR1) expression was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed on a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
製品詳細
BD Pharmingen™
Il8ra;Cxcr1;C-X-C chemokine receptor type 1; CXC-R1; CXCR-1; IL-8R A
Mouse (QC Testing)
Rat LEW, also known as Lewis IgG2b, κ
Mouse CD181 Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2739719
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
サイズ
0.1 mg
カタログ番号
553141
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
サイズ
0.5 mg
カタログ番号
553142
Stain Buffer (FBS) RUO
サイズ
500 mL
カタログ番号
554656
Stain Buffer (BSA) RUO
サイズ
500 mL
カタログ番号
554657
Lysing Buffer RUO
サイズ
100 mL
カタログ番号
555899
Lysing Solution 10X Concentrate RUO (GMP)
サイズ
100 mL
カタログ番号
349202
566383 Rev. 1
抗体の詳細
U45-632
The U45-632 monoclonal antibody specifically binds to mouse CD181, also known as C-X-C chemokine receptor type 1 (CXCR1) or IL-8 receptor α. CD181 is a chemokine receptor of the G-protein coupled receptor 1 family, also known as seven-transmembrane domain receptors. In the mouse, which is not known to express IL-8 (CXCL8), CD181 binds to mouse GCP-2 (CXCL6). Mouse CD181 can also bind to human IL-8. CD181 is expressed by neutrophils, which are activated upon ligand binding. The chemokine receptors CXCR1 and CXCR2 play an important role in mediating neutrophil recruitment and neutrophil-dependent injury in several models of inflammation.
566383 Rev. 1
フォーマットの詳細
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566383 Rev.1
引用＆参考文献
Development References (4)
-
Fan X, Patera AC, Pong-Kennedy A, et al. Murine CXCR1 is a functional receptor for GCP-2/CXCL6 and interleukin-8/CXCL8.. J Biol Chem. 2007; 282(16):11658-66. (Biology). View Reference
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Fu W, Zhang Y, Zhang J, Chen WF. Cloning and characterization of mouse homolog of the CXC chemokine receptor CXCR1.. Cytokine. 2005; 31(1):9-17. (Biology). View Reference
-
Min SH, Wang Y, Gonsiorek W, et al. Pharmacological targeting reveals distinct roles for CXCR2/CXCR1 and CCR2 in a mouse model of arthritis.. Biochem Biophys Res Commun. 2010; 391(1):1080-6. (Biology). View Reference
-
Moepps B, Nuesseler E, Braun M, Gierschik P. A homolog of the human chemokine receptor CXCR1 is expressed in the mouse.. Mol Immunol. 2006; 43(7):897-914. (Biology). View Reference
566383 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
