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Two-color flow cytometric analysis of ZBTB7B (Th-POK) expression in human peripheral blood mononuclear cells. PBMCs were surface stained with Alexa Fluor® 488 Mouse Anti-Human CD4 antibody (Cat. No. 557695). The cells were then washed, fixed, and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725) and stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680) or PE Mouse Anti-Human ZBTB7B (ThPOK) antibody. The two-color flow cytometric contour plots showing the correlated expression of ZBTB7B (ThPOK) [or Ig Isotype Control] versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR Fortessa™ X-20 Flow Cytometer System.
BD Pharmingen™ PE Mouse Anti-Human ZBTB7B (ThPOK)
Regulatory Statusの凡例
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation and Storage
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
関連製品
The 6/hcKrox monoclonal antibody specifically recognizes human Th Inducing POZ-Kruppel Factor (ThPOK) which is also known as, cKrox or hcKrox. ThPOK is encoded by ZBTB7B (Zinc finger and BTB domain-containing protein 7B) and is a member of the POK/ZBT family of transcription regulators that play roles in lymphocyte development and the regulation of type I collagen genes. ThPOK expression is upregulated during the differentiation of CD4+ T helper cells but not CD8+ T cells. It indirectly increases the expression of CD4 in developing T cells by antagonizing Runx-3-mediated CD4 repression. ThPOK is also expressed in NKT and γδ T cells.
Development References (3)
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Heegaard AM, Gehron Robey P, Vogel W. Functional characterization of the human biglycan 5'-flanking DNA and binding of the transcription factor c-Krox. J Bone Miner Res. 1997; 12(12):2050-2060. (Biology). View Reference
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Peterkofsky B, Gosiewska A, Singh K, Pearlman S, Mahmoodian F. Species differences in cis-elements of the proalpha1(I) procollagen promoter and their binding proteins. J Cell Biochem. 1999; 73(3):408-422. (Biology). View Reference
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Widom RL, Culic I, Lee JY, Korn JH. Cloning and characterization of hcKrox, a transcriptional regulator of extracellular matrix gene expression. Gene. 1997; 198(1-2):407-420. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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