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PE Mouse Anti-Human TRIF (TICAM-1)
PE Mouse Anti-Human TRIF (TICAM-1)
Flow cytometric analysis of TRIF (TICAM-1) expression in peripheral blood lymphocytes. Peripheral blood mononuclear cells (PBMCs) were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722). The cells were then washed and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human TRIF (TICAM-1) antibody (Cat. No. 566354; solid line histogram) at 0.5 µg/test. The histogram showing TRIF (TICAM-1) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
PE Mouse Anti-Human TRIF (TICAM-1)

Western blot analysis of TRIF (TICAM-1) expressed by human Raji cells and PBMCs. Proteins from Raji (Burkitt's B cell lymphoma, ATCC CCL-86; Lane 1) cell line or PBMC (Lane 2) lysates were resolved by SDS-PAGE, blotted, and then probed with 1 μg/ml of Purified Mouse Anti-Human TRIF (TICAM-1) antibody followed by HRP-conjugated Goat Anti-Mouse IgG (Cat. No. 554002), and ECL detection reagents. TRIF (TICAM-1) was identified as protein bands of ~95-100 kDa.

Flow cytometric analysis of TRIF (TICAM-1) expression in peripheral blood lymphocytes. Peripheral blood mononuclear cells (PBMCs) were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722). The cells were then washed and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human TRIF (TICAM-1) antibody (Cat. No. 566354; solid line histogram) at 0.5 µg/test. The histogram showing TRIF (TICAM-1) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.

Western blot analysis of TRIF (TICAM-1) expressed by human Raji cells and PBMCs. Proteins from Raji (Burkitt's B cell lymphoma, ATCC CCL-86; Lane 1) cell line or PBMC (Lane 2) lysates were resolved by SDS-PAGE, blotted, and then probed with 1 μg/ml of Purified Mouse Anti-Human TRIF (TICAM-1) antibody followed by HRP-conjugated Goat Anti-Mouse IgG (Cat. No. 554002), and ECL detection reagents. TRIF (TICAM-1) was identified as protein bands of ~95-100 kDa.

製品詳細
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BD Pharmingen™
TICAM1; PRVTIRB; MyD88-3; IIAE6
Human (QC Testing)
Mouse IgG1, κ
Human TRIF Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested), Western blot (Tested During Development)
0.2 mg/ml
AB_2739706
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566354 Rev. 1
抗体の詳細
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U19-709

The U19-709 monoclonal antibody specifically recognizes TIR domain-containing adapter inducing IFN-β (TRIF), which is also known as TIR domain-containing adapter molecule 1 (TICAM-1). TRIF (TICAM-1) is an intracellular adaptor protein that contains a Toll/Interleukin-1 Receptor (TIR) homology domain. Double stranded RNA (dsRNA)-bound Toll-like receptor 3 (TLR3/CD283) or lipopolysaccharide (LPS)-triggered TLR4/CD284 can interact with TRIF (TICAM-1). This leads to downstream signaling cascades involving Interferon regulatory factors (eg, IRF3 or IRF7) or nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) that induce Interferon-beta (IFN- β) expression.

        

566354 Rev. 1
フォーマットの詳細
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566354 Rev.1
引用&参考文献
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Development References (6)

  1. Fitzgerald KA, Rowe DC, Barnes BJ, et al. LPS-TLR4 signaling to IRF-3/7 and NF-kappaB involves the toll adapters TRAM and TRIF.. J Exp Med. 2003; 198(7):1043-55. (Biology). View Reference
  2. Kondo T, Kawai T, Akira S. Dissecting negative regulation of Toll-like receptor signaling.. Trends Immunol. 2012; 33(9):449-58. (Biology). View Reference
  3. Oshiumi H, Matsumoto M, Funami K, Akazawa T, Seya T. TICAM-1, an adaptor molecule that participates in Toll-like receptor 3-mediated interferon-beta induction.. Nat Immunol. 2003; 4(2):161-7. (Biology). View Reference
  4. Seya T, Matsumoto M, Ebihara T, Oshiumi H. Functional evolution of the TICAM-1 pathway for extrinsic RNA sensing.. Immunol Rev. 2009; 227(1):44-53. (Biology). View Reference
  5. Yamamoto M, Sato S, Hemmi H, et al. Role of adaptor TRIF in the MyD88-independent toll-like receptor signaling pathway.. Science. 2003; 301(5633):640-3. (Biology). View Reference
  6. Yamamoto M, Sato S, Mori K, et al. Cutting edge: a novel Toll/IL-1 receptor domain-containing adapter that preferentially activates the IFN-beta promoter in the Toll-like receptor signaling.. J Immunol. 2002; 169(12):6668-72. (Biology). View Reference
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566354 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.