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Multicolor flow cytometric analysis of THEMIS expressed in human PBMC. Human peripheral blood mononuclear cells (PBMC) were isolated from freshly drawn EDTA-treated blood. The cells were fixed and permeabilized using reagents from the BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit (Cat. No. 554714). The cells were then stained with PE Mouse Anti-Human THEMIS (Cat. No. 562588) and PerCP-Cy™5.5 Mouse Anti-Human CD3 (Cat. No. 560835) antibodies. The two-color dot plot was derived from gated events with the forward and side light-scattering characteristics of intact cells. Flow cytometry was performed using a BD™ LSRII Flow Cytometer System.
BD Pharmingen™ PE Mouse anti-Human THEMIS
Regulatory Statusの凡例
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation and Storage
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Cy is a trademark of GE Healthcare.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
The Q13-1103 monoclonal antibody specifically binds to human THEMIS (Thymocyte-expressed molecule involved in selection). The human THEMIS gene encodes a 73-kDa signaling protein that is also known as thymocyte selection pathway associated (TSEPA), signaling phosphoprotein specific for T cells (SPOT) and GRB2-associated protein (GASP). The homologous mouse protein is encoded by the Themis gene. THEMIS mRNA is detected in the thymus, lymph nodes, spleen but not in the bone marrow or nonlymphoid tissues. It is expressed by all thymocytes including double- (high levels) and single-positive αβ T cells, γδ T cells and peripheral T cells (low levels) but not by B cells and NK cells. THEMIS plays a central role in T cell development including positive and negative selection processes in the thymus. It is involved in TCR signaling and gets phosphorylated quickly after TCR stimulation. In the mouse system, Themis-deficient double-positive thymocytes reportedly expressed lower levels of genes involved in later stages of T cell development including those related to cellular metabolism, cell cycle and survival functions. Themis-deficient thymocytes showed impaired selection that lead to the production of fewer mature thymocytes. The Q13-1103 antibody crossreacted with mouse Themis in Western blot testing of cellular lysates but did not crossreact when tested by immunofluorescent staining and flow cytometric analysis of cells.
Development References (4)
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Fu G, Vallée S, Rybakin V, et al. Themis controls thymocyte selection through regulation of T cell antigen receptor-mediated signaling. Nat Immunol. 2009; 10(8):848-856. (Biology). View Reference
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Johnson AL, Aravind L, Shulzhenko N, et al. Themis is a member of a new metazoan gene family and is required for the completion of thymocyte positive selection. Nat Immunol. 2009; 10(8):831-838. (Biology). View Reference
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Lesourne R, Uehara S, Lee J, et al. Themis, a T cell-specific protein important for late thymocyte development. Nat Immunol. 2009; 10(8):840-847. (Biology). View Reference
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Patrick MS, Oda H, Hayakawa K, et al. Gasp, a Grb2-associating protein, is critical for positive selection of thymocytes. Proc Natl Acad Sci U S A. 2009; 106(38):16345-16350. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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