BD Pharmingen™ PE Mouse Anti-Human Light Chain, λ
クローン JDC-12 (RUO)
Flow cytometric analysis of λ light chain expression on human peripheral blood lymphocytes. Whole blood was stained with PE Mouse Anti-Human Light Chain, λ (Cat. No. 555797/562054) and FITC Mouse Anti-Human CD19 (Cat. No. 555412/560994). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color fluorescent dot plot was derived from gated events with the forward and side light-scattering characteristics of intact lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
Flow cytometric analysis of λ light chain expression on human peripheral blood lymphocytes. Whole blood was stained with PE Mouse Anti-Human Light Chain, λ (Cat. No. 555797/562054) and FITC Mouse Anti-Human CD19 (Cat. No. 555412/560994). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color fluorescent dot plot was derived from gated events with the forward and side light-scattering characteristics of intact lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
Preparation and Storage
推奨アッセイ手順
BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
The JDC-12 monoclonal antibody specifically binds to human immunoglobulin light chain, lambda (λ). It does not bind to immunoglobulin κ light chains or heavy chains.
Development References (2)
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Odendahl M, Jacobi A, Hansen A, et al. Disturbed peripheral B lymphocyte homeostasis in systemic lupus erythematosus. J Immunol. 2000; 165(10):5970-5979. (Clone-specific). View Reference
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Sembries S, Pahl H, Stilgenbauer S, Döhner H, Schriever F. Reduced expression of adhesion molecules and cell signaling receptors by chronic lymphocytic leukemia cells with 11q deletion.. Blood. 1999; 93(2):624-31. (Clone-specific: Flow cytometry). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.