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PE Mouse Anti-Human CD79a
PE Mouse Anti-Human CD79a

Flow cytometric analysis of CD79a expression in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were subsequently stained with either PE Mouse Anti-Human CD79a antibody (Cat. No. 563777; solid line histogram) or PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD™ Canto II Flow Cytometer System.

Flow cytometric analysis of CD79a expression in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were subsequently stained with either PE Mouse Anti-Human CD79a antibody (Cat. No. 563777; solid line histogram) or PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD™ Canto II Flow Cytometer System.

製品詳細
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BD Pharmingen™
CD79A; CD79α; Immunoglobulin-associated alpha; Ig-alpha; IGA; Igα; MB-1
Human (QC Testing), Mouse, Rat, Monkey, Pig, Dog, Chicken, Rabbit, Cow, Guinea Pig, Horse (Reported)
Mouse BALB/c IgG1, κ
Human CD79a Peptide
Intracellular staining (flow cytometry) (Routinely Tested)
5 µl/test
V cB018; VI B103
AB_2738423
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
563777 Rev. 1
抗体の詳細
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HM57

The HM57 monoclonal antibody specifically binds to the cytoplasmic domain of CD79a. CD79a is a 47-kDa type 1 transmembrane glycoprotein present on B lymphocytes. CD79a is also referred to as mb-1, IGA and Ig-alpha (Ig-α). It is expressed on B cells at various stages of differentiation, from the pre-B cell stage, probably before expression of cytoplasmic μ chain, to the plasma cell stage, in which it is detected only in the cytoplasm. CD79a associates with CD79b to form part of the B-cell receptor complex. It has been suggested that CD79a may play a role in mediating the transport of IgM to the cell surface. This antibody reportedly may crossreact with monkey, mouse, rat, bovine, canine, chicken, equine, guinea pig, porcine, and rabbit CD79a.

563777 Rev. 1
フォーマットの詳細
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
563777 Rev.1
引用&参考文献
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Development References (6)

  1. Engel P, Wagner N, Tedder TF. CD79 Workshop report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:667-670.
  2. Faldyna M, Sinkora J, Knotigova P, Rehakova Z, Moravkova A, Toman M. Flow cytometric analysis of bone marrow leukocytes in neonatal dogs. Vet Immunol Immunopathol. 2003; 95(3-4):165-176. (Clone-specific: Flow cytometry). View Reference
  3. Jones M, Cordell JL, Beyers AD, Tse AG, Mason DY. Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies. J Immunol. 1993; 150(12):5429-5435. (Clone-specific: Immunohistochemistry, Western blot). View Reference
  4. Mason DY, Cordell JL, Tse AG, et al. The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J Immunol. 1991; 147(11):2474-2482. (Immunogen: Flow cytometry, Immunoaffinity chromatography, Immunofluorescence, Immunohistochemistry, Immunoprecipitation). View Reference
  5. Sakaguchi N, Kashiwamura S, Kimoto M, Thalmann P, Melchers F. B lymphocyte lineage-restricted expression of mb-1, a gene with CD3-like structural properties. EMBO J. 1988; 7(11):3457-3464. (Biology). View Reference
  6. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
すべて表示する (6) 表示項目を減らす
563777 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.