BD Pharmingen™ FITC Rat Anti-Mouse Ig, λ1, λ2, & λ3 Light Chain
クローン R26-46 (RUO)
Flow cytometric analysis of Ig, λ1, λ2, & λ3 light chain expression on mouse splenocytes. B6 splenocytes were costained with PE Rat Anti-Mouse CD45R/B220 (Cat. No. 553089/553090/561878), and either FITC Rat Anti-Mouse Ig, λ1, λ2, & λ3 Light Chain (Cat. No. 553434; Right Panel) or FITC Rat IgG2a, κ Isotype Control (Cat. No. 553929; Left Panel). Two-color dot plots were derived from gated events with the side and forward light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD FACSCanto™ II system.
Flow cytometric analysis of Ig, λ1, λ2, & λ3 light chain expression on mouse splenocytes. B6 splenocytes were costained with PE Rat Anti-Mouse CD45R/B220 (Cat. No. 553089/553090/561878), and either FITC Rat Anti-Mouse Ig, λ1, λ2, & λ3 Light Chain (Cat. No. 553434; Right Panel) or FITC Rat IgG2a, κ Isotype Control (Cat. No. 553929; Left Panel). Two-color dot plots were derived from gated events with the side and forward light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD FACSCanto™ II system.
Flow cytometric analysis of Ig, λ1, λ2, & λ3 light chain expression on mouse splenocytes. B6 splenocytes were costained with PE Rat Anti-Mouse CD45R/B220 (Cat. No. 553089/553090/561878), and either FITC Rat Anti-Mouse Ig, λ1, λ2, & λ3 Light Chain (Cat. No. 553434; Right Panel) or FITC Rat IgG2a, κ Isotype Control (Cat. No. 553929; Left Panel). Two-color dot plots were derived from gated events with the side and forward light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD FACSCanto™ II system.
Flow cytometric analysis of Ig, λ1, λ2, & λ3 light chain expression on mouse splenocytes. B6 splenocytes were costained with PE Rat Anti-Mouse CD45R/B220 (Cat. No. 553089/553090/561878), and either FITC Rat Anti-Mouse Ig, λ1, λ2, & λ3 Light Chain (Cat. No. 553434; Right Panel) or FITC Rat IgG2a, κ Isotype Control (Cat. No. 553929; Left Panel). Two-color dot plots were derived from gated events with the side and forward light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD FACSCanto™ II system.
Preparation and Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
The R26-46 antibody reacts specifically with mouse Igs bearing λ1, λ2, or λ3 light chains. It does not react with κ light chain or heavy chain. Detection of surface immunoglobulin on Ig λ chain-secreting hybridoma cells has been demonstrated with R26-46 mAb.
Development References (2)
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Li Y, Li H, Weigert M. Autoreactive B cells in the marginal zone that express dual receptors.. J Exp Med. 2002; 195(2):181-8. (Clone-specific). View Reference
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Rolink AG, Winkler T, Melchers F, Andersson J. Precursor B cell receptor-dependent B cell proliferation and differentiation does not require the bone marrow or fetal liver environment.. J Exp Med. 2000; 191(1):23-32. (Clone-specific). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.