FITC Rat Anti-Mouse CD107b

BD Pharmingen™ FITC Rat Anti-Mouse CD107b

Name: FITC Rat Anti-Mouse CD107b
Brand: FITC Rat Anti-Mouse CD107b
SKU: 558758

クローン ABL-93 (RUO)

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製品詳細

Brand: BD Pharmingen™

Alternative Name: Lamp2; LAMP-2; LAMP II; Lysosomal-associated membrane protein 2; LGP-B

Reactivity: Mouse (QC Testing)

Isotype: Rat SD, also known as Sprague-Dawley (outbred) IgG2a, λ

Immunogen: Glycoproteins purified from BALB/c mouse embryo 3T3 cell line

Application: Intracellular staining (flow cytometry) (Routinely Tested)

Concentration: 0.5 mg/ml

RRID: AB_397107

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
An isotype control should be used at the same concentration as the antibody of interest.

関連製品

Fixation/Permeabilization Kit RUO

サイズ 250 Tests カタログ番号 554714

558758 Rev. 8

抗体の詳細

ABL-93

The ABL-93 monoclonal antibody specifically recognizes CD107b which is also known as Lysosome-associated membrane protein 2 (LAMP2/LAMP-2), Lysosomal membrane glycoprotein type B (LGP-B), and Mac-3. CD107b is a type I lysosomal transmembrane glycoprotein that serves as a useful marker to distinguish lysosomes from other cellular organelles. Mouse CD107b consists of a ~40-kDa core protein which is heavily glycosylated to form heterogeneous mature glycoproteins of 110-120 kDa. It is principally expressed in epithelial cells and macrophages in a variety of organs in normal and Beige mutant mice.

558758 Rev. 8

フォーマットの詳細

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: FITC

励起源: Blue 488 nm

最大励起波長: 494 nm

最大蛍光波長: 518 nm

558758 Rev.8

引用＆参考文献

View product citations for antibody "558758" on CiteAb

Development References (3)

Cha Y, Holland SM, August JT. The cDNA sequence of mouse LAMP-2. Evidence for two classes of lysosomal membrane glycoproteins. J Biol Chem. 1990; 265(9):5008-5013. (Biology). View Reference
Chen JW, Chen GL, D'Souza MP, Murphy TL, August JT. Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2. Biochem Soc Symp. 1986; 51:97-112. (Biology). View Reference
Chen JW, Murphy TL, Willingham MC, Pastan I, August JT. Identification of two lysosomal membrane glycoproteins. J Cell Biol. 1985; 101(1):85-95. (Immunogen). View Reference

558758 Rev. 8

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.