Preparation and Storage
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BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Product Notices
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Alexa Fluor® is a registered trademark of Life Technologies Corporation.
- BD Horizon Brilliant Violet 650 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
関連製品
The SGP1 monoclonal antibody specifically recognizes human ErbB3 (also known as HER-3), a 160-kDa glycoprotein that is a member of the epidermal growth factor receptor or ErbB family of receptor tyrosine kinases. Other members of the family include the epidermal growth factor receptor (EGFR, ErbB-1, HER1), ErbB-2 (Neu, HER2), and ErbB-4 (HER4). Members of this receptor family mediate the proliferation and differentiation of normal cells. They have a common structure consisting of an extracellular ligand-binding domain, a transmembrane region, and a cytoplasmic region that has sequence homology to tyrosine kinases, which is inactive in ErbB3. ErbB3 is expressed on neurons and in tissues from the digestive, urinary and respiratory tracts, the circulatory system, and female and male reproductive organs. It is overexpressed in a variety of tumors and is undetectable in hematopoietic tissue and cell lines derived from hematopoietic tumors. ErbB3 is able to form heterodimers with other ErbB family members that have active tyrosine kinases. This interaction is able to mediate signal transduction upon binding of ErbB3 to its ligand neuregulin, a cell adhesion molecule that is involved in development of the heart and nervous system.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
Development References (4)
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Münster PN, Marchion DC, Basso AD, Rosen N. Degradation of HER2 by ansamycins induces growth arrest and apoptosis in cells with HER2 overexpression via a HER3, phosphatidylinositol 3'-kinase-AKT-dependent pathway.. Cancer Res. 2002; 62(11):3132-7. (Biology). View Reference
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Rajkumar T, Gullick WJ. A monoclonal antibody to the human c-erbB3 protein stimulates the anchorage-independent growth of breast cancer cell lines.. Br J Cancer. 1994; 70(3):459-65. (Clone-specific: Flow cytometry, Functional assay). View Reference
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Rajkumar T1, Majhi U, Malligarjuna V, Gullick W.. Prevalence of C-erbb3 expression in squamous-cell carcinomas of the cervix as determined by the monoclonal-antibody rtj2.. Int J Oncol. 1995; 6(1):105-109. (Immunogen: Immunohistochemistry, Immunoprecipitation, Western blot). View Reference
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Wang H, Jin Y, Reddy MV, et al. Genetically dependent ERBB3 expression modulates antigen presenting cell function and type 1 diabetes risk.. PLoS ONE. 2010; 5(7):e11789. (Biology: Flow cytometry). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.