BV605 Mouse Anti-Human CD5

This on-demand reagent will be transitioning to a standard catalog equivalent in April 2024. Replacement SKU [569239] is available now. More Info

BD OptiBuild™ BV605 Mouse Anti-Human CD5

Name: BV605 Mouse Anti-Human CD5
Brand: BD OptiBuild™
SKU: 742550

クローン L17F12

(RUO)

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製品詳細

Brand: BD OptiBuild™

Alternative Name: LEU1; Leu-1; Lymphocyte antigen T1; T1; LY1; Tp67

Reactivity: Human (Tested in Development)

Isotype: Mouse BALB/c IgG2a, κ

Immunogen: Human T-cell acute lymphoblastic leukemia (T-ALL) cells

Application: Flow cytometry (Qualified)

Concentration: 0.2 mg/ml

Workshop Number: II T7

RRID: AB_2740860

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV605 under optimal conditions that minimize unconjugated dye and antibody.

推奨アッセイ手順

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

This antibody was developed for use in flow cytometry.
The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
Researchers should determine the optimal concentration of this reagent for their individual applications.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
BD Horizon Brilliant Violet 605 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.

関連製品

Human BD Fc Block™ RUO

サイズ 50 µg カタログ番号 564219

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

Stain Buffer (BSA) RUO

サイズ 500 mL カタログ番号 554657

Brilliant Stain Buffer RUO

サイズ 100 Tests カタログ番号 563794

Lysing Buffer RUO

サイズ 100 mL カタログ番号 555899

Lysing Solution 10X Concentrate RUO (GMP)

サイズ 100 mL カタログ番号 349202

詳細を表示

742550 Rev. 1

抗体の詳細

L17F12

The L17F12 monoclonal antibody specifically binds to CD5 which is also known as Lymphocyte antigen T1 (T1) or LEU1/Leu-1. CD5 is an ~67 kDa single-chain, type 1 transmembrane glycoprotein expressed on most thymocytes, the majority of peripheral T lymphocytes and a subpopulation of B cells. CD5+ B cells produce polyreactive antibodies (mostly IgM). This CD5-specific antibody also reacts with many T-cell leukemias and lymphomas and most Ig+ B-cell chronic lymphoblastic leukemia (B-CLL) cells. CD72 has been shown to be the natural ligand for CD5.

This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.

742550 Rev. 1

フォーマットの詳細

BV605

The BD Horizon Brilliant Violet™ 605 (BV605) dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 605-nm. BV605, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 610-nm (e.g., a 610/20-nm bandpass filter). The acceptor dye can be excited by the yellow-green (561-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: BV605

励起源: Violet 405 nm

最大励起波長: 407 nm

最大蛍光波長: 605 nm

742550 Rev.1

引用＆参考文献

View product citations for antibody "742550" on CiteAb

Development References (4)

Engleman EG, Warnke R, Fox RI, Dilley J, Benike CJ, Levy R. Studies of a human T lymphocyte antigen recognized by a monoclonal antibody.. Proc Natl Acad Sci USA. 1981; 78(3):1791-5. (Immunogen: Cell separation, Cytotoxicity, Depletion, Flow cytometry, Fluorescence activated cell sorting, Functional assay, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay). View Reference
Knowles RW. Immunochemical analysis of the T-cell–specific antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:259-288.
Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:1-560.
Sagawa K, Hagiwara S, Yasuda K, Yokoyama MM. Expression profiles of T cell associated antigens on adult T cell leukemia cells: Results of a workshop study. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:361-369.

すべて表示する (4)

742550 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.