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製品詳細
BD OptiBuild™
FCAR; IgA Fc receptor; FcαR; FcalphaRI; CTB-61M7.2
Human (Tested in Development)
Mouse BALB/c IgG1, κ
Purified Human FcαR
Flow cytometry (Qualified)
0.2 mg/ml
V MA104
AB_2742188
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV510 under optimal conditions that minimize unconjugated dye and antibody.
推奨アッセイ手順
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Product Notices
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Violet 510 is covered by one or more of the following US patents: 8,575,303; 8,354,239.
関連製品
Human BD Fc Block™ RUO
サイズ
50 µg
カタログ番号
564219
Stain Buffer (FBS) RUO
サイズ
500 ML
カタログ番号
554656
Stain Buffer (BSA) RUO
サイズ
500 ML
カタログ番号
554657
Brilliant Stain Buffer RUO
サイズ
100 Tests
カタログ番号
563794
Lysing Buffer RUO
サイズ
100 ML
カタログ番号
555899
Lysing Solution 10X Concentrate RUO (GMP)
サイズ
100 ML
カタログ番号
349202
744375 Rev. 1
抗体の詳細
A59
The A59 monoclonal antibody specifically recognizes CD89. CD89 is the Fc receptor for IgA (FcαR), a 55-75 kDa glycoprotein expressed exclusively on cells of granulocytic and monocyte/macrophage lineages in peripheral blood, but not on lymphocytes. It is also expressed on promyelocytes in bone marrow and in the cytoplasm of fixed monocytes. This mAb does not block IgA binding and is more efficient than native IgA ligands in the isolation of FcαR molecules. FcαR plays a role in triggering phagocytosis and respiratory burst.
The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.
744375 Rev. 1
フォーマットの詳細
BV510
The BD Horizon Brilliant Violet™ 510 (BV510) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye with an excitation maximum (Ex Max) at 327-nm / 405-nm and an emission maximum (Em Max) at 512-nm. BV510, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 510-nm (e.g., a 525/50 bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV510
327 nm, 405 nm
512 nm
744375 Rev.1
引用＆参考文献
View product citations for antibody "744375" on CiteAb
Development References (4)
-
Monteiro RC, Cooper MD, Kubagawa H. Molecular heterogeneity of Fc alpha receptors detected by receptor-specific monoclonal antibodies. J Immunol. 1992; 148(6):1764-1770. (Immunogen: ELISA, Flow cytometry, Immunoprecipitation, Radioimmunoassay). View Reference
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
-
Shen L, Collins JE, Schoenborn MA, Maliszewski CR. Lipopolysaccharide and cytokine augmentation of human monocyte IgA receptor expression and function. J Immunol. 1994; 152(8):4080-4086. (Biology). View Reference
-
Shen L. A monoclonal antibody specific for immunoglobulin A receptor triggers polymorphonuclear neutrophil superoxide release. J Leukoc Biol. 1992; 51(4):373-378. (Biology). View Reference
744375 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.