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BV421 Mouse Anti-Human MSR1 (CD204)
BV421 Mouse Anti-Human MSR1 (CD204)

Flow cytometric analysis using BD OptiBuild™ BV421 Mouse Anti-Human MSR1 (CD204) antibody (Cat. No. 742438; solid line histogram) on MSR1-transfected 293F cells, with Isotype Control (dotted line histogram). Flow cytometry was performed using a BD LSRFortessa™ X-20  Flow Cytometer System.

Flow cytometric analysis using BD OptiBuild™ BV421 Mouse Anti-Human MSR1 (CD204) antibody (Cat. No. 742438; solid line histogram) on MSR1-transfected 293F cells, with Isotype Control (dotted line histogram). Flow cytometry was performed using a BD LSRFortessa™ X-20  Flow Cytometer System.

製品詳細
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BD OptiBuild™
MSR; MSRE; phSR1; phSR2; SCARA1; SR-A; SRA
Human (Tested in Development)
Mouse IgG1, κ
Human MSR1 Recombinant Protein
Flow cytometry (Qualified)
0.2 mg/ml
AB_2740781
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.

推奨アッセイ手順

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  10. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
742438 Rev. 2
抗体の詳細
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U23-56

The U23-56 monoclonal antibody specifically binds to Macrophage scavenger receptor 1 (MSR1) which is also known as CD204, Scavenger receptor A (SRA), Scavenger receptor class A member 1 (SCARA1), or Macrophage acetylated LDL receptor I and II. MSR1 is a trimeric type II transmembrane glycoprotein that belongs to the type 1 class A scavenger receptor family. MSR1 functions as a scavenger receptor that enables mononuclear phagocytes to take up and respond to a variety of negatively charged macromolecules including oxidized and acetylated low density lipoproteins. MSR1 also functions in innate immunity as a pattern recognition receptor. MSR1 allows macrophages to recognize and respond to microbial products, such as lipopolysaccharide and polyribonucleic acids, by the production of cytokines and inflammatory mediators. MSR1 also reportedly serves as a coreceptor that regulates the signaling of some Toll-like receptors including TLR-3, TLR-4, and TLR-9. MSR1 has been associated with several diseases including atherosclerosis, Alzheimer's disease, ischemic injury, and sepsis. The U23-56 antibody was tested by flow cytometric and Western blot analyses of MSR1-transfected and untransfected cells to verify its specificity.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

742438 Rev. 2
フォーマットの詳細
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
742438 Rev.2
引用&参考文献
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Development References (4)

  1. Gough PJ, Greaves DR, Gordon S. A naturally occurring isoform of the human macrophage scavenger receptor (SR-A) gene generated by alternative splicing blocks modified LDL uptake.. J Lipid Res. 1998; 39(3):531-43. (Biology). View Reference
  2. Higashi-Kuwata N, Jinnin M, Makino T, et al. Characterization of monocyte/macrophage subsets in the skin and peripheral blood derived from patients with systemic sclerosis.. Arthritis Res Ther. 2010; 12(4):R128. (Biology). View Reference
  3. Hongo S, Watanabe T, Arita S, et al. Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages.. Am J Physiol Endocrinol Metab. 2009; 297(2):E474-82. (Biology). View Reference
  4. Zola H, Swart B, Nicholson I, Voss E. CD204. In: Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007:347-348.
すべて表示する (4) 表示項目を減らす
742438 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.