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BV421 Mouse Anti-Human MICA
BV421 Mouse Anti-Human MICA

Flow cytometric analysis of MICA expression on human adenocarcinoma cells. HeLa cells (ATCC CCL-2) were stained with either or BD OptiBuild™ BV421 Mouse Anti-Human MICA (Cat. No. 749779, solid line histogram) or BD Horizon™ BV421 Mouse IgGb, κ Isotype Control (Cat. No. 562748; dashed line histogram) at 0.25 µg/test. The fluorescence histogram showing MICA (or Ig Isotype control) staining was derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.

The above is qualification data only and does not represent a specific OptiBuild™ lot.

Flow cytometric analysis of MICA expression on human adenocarcinoma cells. HeLa cells (ATCC CCL-2) were stained with either or BD OptiBuild™ BV421 Mouse Anti-Human MICA (Cat. No. 749779, solid line histogram) or BD Horizon™ BV421 Mouse IgGb, κ Isotype Control (Cat. No. 562748; dashed line histogram) at 0.25 µg/test. The fluorescence histogram showing MICA (or Ig Isotype control) staining was derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.

The above is qualification data only and does not represent a specific OptiBuild™ lot.

製品詳細
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BD OptiBuild™
MHC class I chain-related protein A; MHC class I related chain A; MIC-A; PERB11.1; stress inducible class I homolog
Human (Tested in Development)
Mouse IgG2b, κ
Human MICA Transfected Cell Line
Flow cytometry (Qualified)
0.2 mg/ml
AB_2874033
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


調製と保管

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.

推奨アッセイ手順

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

製品通知

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  10. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
749779 Rev. 4
抗体の詳細
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159227

The 159227 monoclonal antibody specifically recognizes MHC class I polypeptide-related sequence A (MICA) which is also known as MIC-A, MHC class I chain-related protein A, or stress inducible class I homolog. This ~70 kDa single-pass type I transmembrane glycoprotein is encoded by MICA (major histocompatibility complex class I chain-related gene A) which is a highly polymorphic gene. MICA is very homologous to another protein, MICB. Both MICA and MICB are homologous to major histocompatibility complex (MHC) class I molecules although they lack association with β2 microglobulin. MICA and MICB each contain three extracellular Ig domains but have no capacity to bind peptides. MICA is expressed on some gut epithelial cells. Its expression by other epithelial cells and cell types, including fibroblasts and endothelial cells, is induced by stress, eg, stress caused by bacterial and viral infections, autoimmunity, transplantation rejection, or cellular transformation. MICA and MICB are ligands for NKG2D (CD314), an activating receptor expressed by natural killer (NK) cells, γδ T cells, CD8+ αβ T cells, and some CD4+ αβ T cells.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

749779 Rev. 4
フォーマットの詳細
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
BV421
Violet 405 nm
407 nm
423 nm
749779 Rev.4
引用&参考文献
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開発者向け参考資料 (3)

  1. Gómez-Lomelí P, Bravo-Cuellar A, Hernández-Flores G, et al. Increase of IFN-γ and TNF-α production in CD107a + NK-92 cells co-cultured with cervical cancer cell lines pre-treated with the HO-1 inhibitor.. Cancer Cell Int. 2014; 14(1):100. (Clone-specific: Flow cytometry). 参考文献を見る
  2. Niu C, Jin H, Li M, et al. Low-dose bortezomib increases the expression of NKG2D and DNAM-1 ligands and enhances induced NK and γδ T cell-mediated lysis in multiple myeloma.. Oncotarget. 2017; 8(4):5954-5964. (Clone-specific: Flow cytometry). 参考文献を見る
  3. Robinet P, Baychelier F, Fontaine T, et al. A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells.. J Immunol. 2014; 192(11):5332-42. (Clone-specific: Flow cytometry). 参考文献を見る
749779 Rev. 4

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.