Preparation and Storage
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For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Product Notices
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Ultraviolet 661 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,575,303; 8,354,239.
関連製品
The KJ23 monoclonal antibody specifically recognizes Vβ 17[a] T-cell Receptor (TCR) of mice having the a haplotype (eg,C57L, SJL, SWR) of the Tcrb gene complex. Strains having the b (eg, A, AKR, BALB/c, CBA,C3H/He, C57BL, C58, DBA/1, DBA/2) Tcrb haplotype do not express functional Vβ 17 TCR, and the Tcrb-V17 gene locus is deleted in mice having the c (eg, RIII) haplotype. Vβ 17[a] TCR-bearing Tlymphocytes are clonally eliminated in mice expressing I-E (eg, C57BR). KJ23 antibody also recognizes two phenotypic variants of the Vβ 17[a] TCR: Vβ 17[a2] expressed in a variety of wild-derivedmouse strains and Vβ 17[a(cz)] expressed in Mtv-free CZ mice. The effects of Mtv-encoded superantigens upon Vβ 17[a] TCR-bearing T cells has been reviewed. Plate-bound KJ23 antibody activates Vβ 17[a] TCR-bearing T cells, and injection of the antibody can deplete Vβ 17[a]-bearing Tcells.
The antibody was conjugated to BD Horizon™ BUV661 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 661-nm. BD Horizon Brilliant BUV661 can be excited by the ultraviolet laser (355 nm) and detected with a 670/25 filter and a 630 nm LP. Due to cross laser excitation of this dye, there may be significant spillover into channels detecting APC-like emissions (eg, 670/25-nm filter).
Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV661 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. Different BUV661 reagents (eg, CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone-specific compensation controls when using these reagents.
Development References (8)
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Cazenave PA, Marche PN, Jouvin-Marche E, et al. V beta 17 gene polymorphism in wild-derived mouse strains: two amino acid substitutions in the V beta 17 region greatly alter T cell receptor specificity. Cell. 1990; 63(4):717-728. (Biology). View Reference
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Haqqi TM, Banerjee S, Anderson GD, David CS. RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes. J Exp Med. 1989; 169(6):1903-1909. (Biology). View Reference
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Kappler JW, Roehm N, Marrack P. T cell tolerance by clonal elimination in the thymus. Cell. 1987; 49(2):273-280. (Biology). View Reference
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Kappler JW, Wade T, White J, . A T cell receptor V beta segment that imparts reactivity to a class II major histocompatibility complex product. Cell. 1987; 49(2):263-271. (Immunogen). View Reference
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Katz JD, Lebow LT, Bonavida B. The in vivo depletion of V beta 17a+ T cells results in the inhibition of reticulum cell sarcoma growth in SJL/J mice. Evidence for the use of anticlonotypic antibody therapy in the control of malignancy. J Immunol. 1989; 143(4):1387-1395. (Biology). View Reference
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Ramsdell F, Lantz T, Fowlkes BJ. A nondeletional mechanism of thymic self tolerance. Science. 1989; 246(4933):1038-1041. (Biology). View Reference
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Tomonari K, Fairchild S, Rosenwasser OA. Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection. Immunol Rev. 1993; 131:131-168. (Biology). View Reference
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Wade T, Bill J, Marrack PC, Palmer E, Kappler JW. Molecular basis for the nonexpression of V beta 17 in some strains of mice. J Immunol. 1988; 141(6):2165-2167. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.