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      BUV496 Mouse Anti-Human CD44
      741151Image1.png

      Multiparameter flow cytometric analysis using BD OptiBuild™ BUV496 Mouse Anti-Human CD44 antibody (Cat. No. 741151; Right Plot) on Human peripheral blood, with corresponding IgG Isotype Control (Cat. No. 612958; Left Plot). Flow cytometry was performed using a BD LSRFortessa™ X-20  Flow Cytometer System.

      741151_Image2.png

      Multiparameter flow cytometric analysis using BD OptiBuild™ BUV496 Mouse Anti-Human CD44 antibody (Cat. No. 741151; Right Plot) on Human peripheral blood, with corresponding IgG Isotype Control (Cat. No. 612958; Left Plot). Flow cytometry was performed using a BD LSRFortessa™ X-20  Flow Cytometer System.

      741151Image1.png 741151_Image2.png Blue-Cap_UV.png

      Multiparameter flow cytometric analysis using BD OptiBuild™ BUV496 Mouse Anti-Human CD44 antibody (Cat. No. 741151; Right Plot) on Human peripheral blood, with corresponding IgG Isotype Control (Cat. No. 612958; Left Plot). Flow cytometry was performed using a BD LSRFortessa™ X-20  Flow Cytometer System.

      Multiparameter flow cytometric analysis using BD OptiBuild™ BUV496 Mouse Anti-Human CD44 antibody (Cat. No. 741151; Right Plot) on Human peripheral blood, with corresponding IgG Isotype Control (Cat. No. 612958; Left Plot). Flow cytometry was performed using a BD LSRFortessa™ X-20  Flow Cytometer System.

      製品詳細
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      BD OptiBuild™
      Phagocytic glycoprotein 1; Pgp-1; H-CAM; Hermes; ECMR III; HUTCH-1
      Human (Tested in Development)
      Mouse IgG2b, κ
      Flow cytometry (Qualified)
      0.2 mg/ml
      VI A092
      960
      AB_2870729
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation and Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV496 under optimal conditions that minimize unconjugated dye and antibody.
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      推奨アッセイ手順

      For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

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      Product Notices

      1. This antibody was developed for use in flow cytometry.
      2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      3. Researchers should determine the optimal concentration of this reagent for their individual applications.
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
      8. BD Horizon Brilliant Ultraviolet 496 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,575,303; and 8,354,239.
      9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      741151 Rev. 3
      抗体の詳細
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      G44-26

      The G44-26 monoclonal antibody specifically binds to the 80-95 kDa glycosylated type I transmembrane protein, CD44, also known as phagocytic glycoprotein-1 (Pgp-1). CD44 is the receptor for hyaluronic acid. CD44 is expressed on leucocytes, erythrocytes, epithelial cells and weakly on platelets. CD44 is also called extracellular matrix receptor type III and has functional roles in cell migration, lymphocyte homing and adhesion during hematopoiesis and lymphocyte activation. This antibody recognizes epitope 1 of CD44 antigen according to the HLDA workshop studies.

      The antibody was conjugated to BD Horizon™ BUV496 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 496-nm. BD Horizon BUV496 can be excited by the ultraviolet laser (355 nm) and detected with a 515/30 nm filter with a 450LP. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into the channel detecting BD Horizon V500 or BV510 (eg, 525/40-nm filter). However, the spillover can be corrected through compensation as with any other dye combination.

      741151 Rev. 3
      フォーマットの詳細
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      BUV496
      The BD Horizon Brilliant™ Ultraviolet 496 (BUV496) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This tandem fluorochrome is comprised of a BUV395 donor with an excitation maximum (Ex Max) of 350-nm and an acceptor dye with an emission maximum (Em Max) at 496-nm. BUV496, driven by BD innovation, is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 500-nm (e.g., 515/30-nm bandpass filter). The acceptor dye can be excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BUV496
      350 nm
      496 nm
      741151 Rev.3
      引用＆参考文献
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      View product citations for antibody "741151" on CiteAb

      Development References (5)

      1. Galandrini R, Galluzzo E, Albi N, Grossi CE, Velardi A. Hyaluronate is costimulatory for human T cell effector functions and binds to CD44 on activated T cells. J Immunol. 1994; 153(1):21-31. (Biology). View Reference
      2. Günthert U. CD44: a multitude of isoforms with diverse functions. Curr Top Microbiol Immunol. 1993; 184:47-63. (Biology). View Reference
      3. Patel DD, Liao HX, Haynes BF. CD44 workshop panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:373-375.
      4. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
      5. Stamenkovic I, Amiot M, Pesando JM, Seed B. A lymphocyte molecule implicated in lymph node homing is a member of the cartilage link protein family. Cell. 1989; 56(6):1057-1062. (Biology). View Reference
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      741151 Rev. 3

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.