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Biotin Rat Anti-Mouse CD150 (SLAM)
Biotin Rat Anti-Mouse CD150 (SLAM)

Multicolor flow cytometric analysis of CD150 (SLAM) expression by adult mouse spleen cells. BALB/c spleen cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were stained with BD Horizon™ BUV395 Rat Anti-Mouse CD45R/B220 (Cat. No. 563793) and either Biotin Rat IgG2a, κ Isotype Control (Cat. No. 553928; Left Plot) or Biotin Rat Anti-Mouse CD150 (SLAM) antibody (Cat. No. 567659; Right Plot) at 1 µg/test followed by PE Streptavidin (Cat. No. 554061). BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. A bivariate pseudocolor density plot showing CD150 (SLAM) expression (or Ig Isotype control staining) versus CD45R/B220 was derived from gated events with the forward and side-light scattering characteristics of viable (7-AAD-negative) lymphocytes. Flow cytometry and data analysis was performed using a BD™ LSR II Flow Cytometer System and FloJo™ software. Data shown on this Technical Data Sheet are not lot specific.

Multicolor flow cytometric analysis of CD150 (SLAM) expression by adult mouse spleen cells. BALB/c spleen cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were stained with BD Horizon™ BUV395 Rat Anti-Mouse CD45R/B220 (Cat. No. 563793) and either Biotin Rat IgG2a, κ Isotype Control (Cat. No. 553928; Left Plot) or Biotin Rat Anti-Mouse CD150 (SLAM) antibody (Cat. No. 567659; Right Plot) at 1 µg/test followed by PE Streptavidin (Cat. No. 554061). BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. A bivariate pseudocolor density plot showing CD150 (SLAM) expression (or Ig Isotype control staining) versus CD45R/B220 was derived from gated events with the forward and side-light scattering characteristics of viable (7-AAD-negative) lymphocytes. Flow cytometry and data analysis was performed using a BD™ LSR II Flow Cytometer System and FloJo™ software. Data shown on this Technical Data Sheet are not lot specific.

製品詳細
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BD Pharmingen™
SLAM; Slamf1; Signaling lymphocytic activation molecule family member 1
Mouse (QC Testing)
Rat IgG2a, κ
Mouse CD150 Recombinant Protein
Flow cytometry (Routinely Tested)
0.5 mg/ml
27218
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation and Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).

関連製品

Stain Buffer (BSA) RUO
サイズ 500 mL カタログ番号 554657
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Stain Buffer (FBS) RUO
サイズ 500 mL カタログ番号 554656
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Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
サイズ 0.5 mg カタログ番号 553142
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Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
サイズ 0.1 mg カタログ番号 553141
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BUV395 Rat Anti-Mouse CD45R/B220 RUO
サイズ 50 µg カタログ番号 563793
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Biotin Rat IgG2a κ Isotype Control RUO
サイズ 0.25 mg カタログ番号 553928
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567659 Rev. 1
抗体の詳細
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Q38-480

The Q38-480 monoclonal antibody specifically binds to mouse CD150, also known as SLAM (signaling lymphocyte activation molecule). CD150 is a type 1 transmembrane glycoprotein that is a member of the CD2 subfamily of the Ig superfamily. It is encoded by the Slamf1 (signaling lymphocytic activation molecule family member 1) gene. CD150 is differentially expressed on subsets of thymocytes, T and B lymphocytes, dendritic cells, macrophages, and endothelial cells. SLAM plays multiple roles in innate and adaptive immunity serving as an adhesion molecule and/or coreceptor. CD150-mediated costimulation of TCR-activated T cells reportedly results in the increased production of IFN-γ by Th1 cells and is required for IL-4 production by T follicular helper cells. CD150 also plays important roles in hematopoietic cell developmental pathways. CD150 is differentially expressed by self-renewing  adult hematopoietic stem cells (HSC) whereas non-multipotent hematopoietic progenitor cells are CD150-. Utilizing additional cell surface markers, lineage-negative CD150+CD48-CD41- cell fractions are reported to be highly enriched for adult HSC.

567659 Rev. 1
フォーマットの詳細
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
567659 Rev.1
引用&参考文献
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Development References (5)

  1. Castro AG, Hauser TM, Cocks BG, et al. Molecular and functional characterization of mouse signaling lymphocytic activation molecule (SLAM): differential expression and responsiveness in Th1 and Th2 cells.. J Immunol. 1999; 163(11):5860-70. (Biology). View Reference
  2. Guo H, Ma O, Friedman AD. The Cebpa +37-kb enhancer directs transgene expression to myeloid progenitors and to long-term hematopoietic stem cells.. J Leukoc Biol. 2014; 96(3):419-26. (Clone-specific: Flow cytometry). View Reference
  3. Kiel MJ, Yilmaz OH, Iwashita T, Terhorst C, Morrison SJ. SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells. Cell. 2005; 121(7):1109-1121. (Biology). View Reference
  4. Wang N, Satoskar A, Faubion W, et al. The cell surface receptor SLAM controls T cell and macrophage functions. J Exp Med. 2004; 199(9):1255-1264. (Biology). View Reference
  5. Yusuf I, Kageyama R, Monticelli L, et al. Germinal center T follicular helper cell IL-4 production is dependent on signaling lymphocytic activation molecule receptor (CD150). J Immunol. 2010; 185(1):190-202. (Biology). View Reference
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567659 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.