Preparation and Storage
推奨アッセイ手順
For the sandwich rat IgG2a ELISA, biotinylated mAb RG7/1.30 is optimal for detection with purified anti-rat IgG2a mAb B46-7 (Cat. No. 553918) for capture. Biotin-conjugated RG7/1.30 mAb may be used as a secondary reagent in immunofluorescent staining. RG7/1.30 antibody is effective for detection of cell-surface or intracellular Ig by immunofluorescent staining with flow cytometric analysis. For flow cytometric detection of intracytoplasmic Ig2a, we recommend FITC-conjugated RG7/1.30 mAb (Cat. No. 553896). For immunohistochemical staining, we recommend the use of biotinylated anti-rat IgG1/2a mAb G28-5 in our special formulation for immunohistochemistry, Cat. No. 550325.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
関連製品
The Rg7/1.30 antibody reacts specifically with the Fc region of rat IgG2a. It does not react with other Ig isotypes.
Development References (1)
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Springer TA, Bhattacharya A, Cardoza JT, Sanchez-Madrid F. Monoclonal antibodies specific for rat IgG1, IgG2a, and IgG2b subclasses, and kappa chain monotypic and allotypic determinants: reagents for use with rat monoclonal antibodies. Hybridoma. 1982; 1(3):257-273. (Immunogen). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.