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Multicolor flow cytometric analysis of LPAM-1 expression on C57BL/6 mouse bone marrow cells. Bone marrow cells were stained with FITC Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553087/553088/561877) and with either APC Rat IgG2a, κ Isotype Control (Cat. No. 553932; Left Panel) or APC Rat Anti-Mouse LPAM-1 (Cat. No. 562376; Right Panel). Two-color flow cytometric dot plots show the correlated expression patterns of LPAM-1 (or Ig isotype control staining) versus CD45R/B220 for gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
BD Pharmingen™ APC Rat Anti-Mouse LPAM-1
Regulatory Statusの凡例
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation and Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
The DATK32 monoclonal antibody specifically binds to an epitope specific for the α4β7 integrin heterodimer. α4β7 (LPAM-1) is expressed on most mature lymphocytes and on small subsets of thymic and bone marrow cells. It interacts with several ligands, including VCAM-1 (CD106), fibronectin, and MAdCAM-1. DATK32 antibody induces α4β7-dependent lymphocyte aggregation, but it inhibits other α4β7- mediated lymphocyte adhesion events, including binding to fibronectin, MAdCAM-1, and VCAM-1 (CD106).
Development References (5)
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Andrew DP, Berlin C, Honda S, et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J Immunol. 1994; 153(9):3847-3861. (Immunogen: Blocking, Inhibition). View Reference
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Andrew DP, Rott LS, Kilshaw PJ, Butcher EC. Distribution of alpha 4 beta 7 and alpha E beta 7 integrins on thymocytes, intestinal epithelial lymphocytes and peripheral lymphocytes. Eur J Immunol. 1996; 26(4):897-905. (Clone-specific: Blocking). View Reference
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Berlin C, Berg EL, Briskin MJ, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell. 1993; 74(1):185-195. (Clone-specific: Blocking). View Reference
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Hamann A, Andrew DP, Jablonski-Westrich D, Holzmann B, Butcher EC. Role of alpha 4-integrins in lymphocyte homing to mucosal tissues in vivo. J Immunol. 1994; 152(7):3282-3293. (Clone-specific). View Reference
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Kilshaw PJ, Murant SJ. Expression and regulation of beta 7(beta p) integrins on mouse lymphocytes: relevance to the mucosal immune system. Eur J Immunol. 1991; 21(10):2591-2597. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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