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Multicolor flow cytometric analysis of TCR βF1 expression by human peripheral blood lymphocytes. Whole blood was surface stained with FITC Mouse Anti-Human TCR αβ (Cat. No. 555547; Top Plots) and PE Mouse Anti-Human TCR γδ (Cat. No. 561994; Bottom Plots) antibodies. Erythrocytes were lysed and leucocytes were fixed with 1x BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049). After permeabilization with BD™ Phosflow Perm Buffer I (Cat. No. 557885), the cells were then stained with either APC Mouse IgG1, κ Isotype Control (Cat. No. 554681; Left Plots) or APC Mouse Anti-Human TCR βF1 antibody (Cat. No. 566052; Right Plots). Two color contour plots showing the correlated expression of TCR βF1 (or Ig Isotype control staining) versus TCR αβ or TCR γδ were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
BD Pharmingen™ APC Mouse Anti-Human TCR βF1
Regulatory Statusの凡例
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation and Storage
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
The 8A3 monoclonal antibody is also known as the β Framework 1 (βF1) antibody. After treatment of lymphoid cells with permeabilization reagents, this antibody recognizes a common epitope on the β chain of the T Cell Receptor (TCR) for antigen which is expressed by thymocytes and peripheral T lymphocytes. It does not react with γδ TCR-bearing T cells. The 8A3 antibody does not stain the surface of living cells. This suggests that the TCR β constant region epitope recognized by the 8A3 antibody is normally hidden on viable cells.
Development References (5)
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Asnafi V, Beldjord K, Boulanger E, et al. Analysis of TCR, pT alpha, and RAG-1 in T-acute lymphoblastic leukemias improves understanding of early human T-lymphoid lineage commitment. Blood. 2003; 101(7):2693-2703. (Clone-specific: Flow cytometry). View Reference
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Brenner MB, McLean J, Scheft H, Warnke RA, Jones N, Strominger JL. Characterization and expression of the human alpha beta T cell receptor by using a framework monoclonal antibody. J Immunol. 1987; 138(5):1502-1509. (Immunogen: Flow cytometry, Fluorescence microscopy, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western blot). View Reference
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Fraser JD, Strominger JL. A solubilized T-cell receptor from a human leukemia cell line binds to a ligand in the absence of MHC products.. Immunogenetics. 1988; 28(2):108-16. (Clone-specific: Functional assay). View Reference
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Ramiro AR, Trigueros C, Márquez C, San Millán JL, Toribio ML. Regulation of pre-T cell receptor (pT alpha-TCR beta) gene expression during human thymic development. J Exp Med. 1996; 184(2):519-530. (Clone-specific: Flow cytometry). View Reference
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Torres PS, Alcover A, Zapata DA, et al. TCR dynamics in human mature T lymphocytes lacking CD3 gamma. J Immunol. 2003; 170(12):5947-5955. (Clone-specific: Flow cytometry, Fluorescence microscopy, Immunofluorescence). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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