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BD Pharmingen™ APC Mouse Anti-Human CD45RA
クローン HI100 (RUO)
Multicolor flow cytometric analysis of CD45RA expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD45RO antibody (Cat. No. 555493/561889), and with either APC Mouse IgG2b, κ Isotype Control (Cat. No. 555745; Left Plot) or APC Mouse Anti-Human CD45RA antibody (Cat. No. 550855/561884; Right Plot). The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Bivariate pseudocolor density plots showing the correlated expression of CD45RA (or Ig Isotype control) versus CD45RO was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.
Multicolor flow cytometric analysis of CD45RA expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD45RO antibody (Cat. No. 555493/561889), and with either APC Mouse IgG2b, κ Isotype Control (Cat. No. 555745; Left Plot) or APC Mouse Anti-Human CD45RA antibody (Cat. No. 550855/561884; Right Plot). The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Bivariate pseudocolor density plots showing the correlated expression of CD45RA (or Ig Isotype control) versus CD45RO was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.
Multicolor flow cytometric analysis of CD45RA expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD45RO antibody (Cat. No. 555493/561889), and with either APC Mouse IgG2b, κ Isotype Control (Cat. No. 555745; Left Plot) or APC Mouse Anti-Human CD45RA antibody (Cat. No. 550855/561884; Right Plot). The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Bivariate pseudocolor density plots showing the correlated expression of CD45RA (or Ig Isotype control) versus CD45RO was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.
Multicolor flow cytometric analysis of CD45RA expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD45RO antibody (Cat. No. 555493/561889), and with either APC Mouse IgG2b, κ Isotype Control (Cat. No. 555745; Left Plot) or APC Mouse Anti-Human CD45RA antibody (Cat. No. 550855/561884; Right Plot). The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Bivariate pseudocolor density plots showing the correlated expression of CD45RA (or Ig Isotype control) versus CD45RO was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.
製品詳細
BD Pharmingen™
CD45R; PTPRC; LCA; Leukocyte common antigen
Human (QC Testing)
Mouse IgG2b, κ
Flow cytometry (Routinely Tested)
20 µl
IV N906
AB_398468
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
推奨アッセイ手順
BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
550855 Rev. 7
抗体の詳細
HI100
The HI100 monoclonal antibody specifically binds to the 220 kDa isoform of the human leukocyte common antigen, CD45RA. CD45RA is expressed on approximately 40-50% of peripheral CD4+ T cells, 50% of peripheral CD8+ T cells and on a portion of B cells and monocytes. The CD45RA antigen is expressed by naïve and activated T cells. CD45RA-specific antibodies are useful for the study of the suppressor/inducer subpopulation of CD4+ lymphocytes.
550855 Rev. 7
フォーマットの詳細
APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC
651 nm
660 nm
550855 Rev.7
引用＆参考文献
View product citations for antibody "550855" on CiteAb
Development References (4)
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Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
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Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
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Koristka S, Cartellieri M, Theil A, et al. Retargeting of human regulatory T cells by single-chain bispecific antibodies.. J Immunol. 2012; 188(3):1551-8. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). View Reference
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Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
550855 Rev. 7
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.