APC Mouse Anti-Human CD181

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BD Pharmingen™ APC Mouse Anti-Human CD181

Name: APC Mouse Anti-Human CD181
Brand: BD Pharmingen™
SKU: 551080

クローン 5A12

(RUO)

すべてのフォーマットを表示

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Scientific Resources

Profile of peripheral blood granulocytes analyzed by flow cytometry.

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製品詳細

Brand: BD Pharmingen™

Alternative Name: CXCR1, IL-8RA, CD128a

Reactivity: Human (QC Testing)

Isotype: Mouse IgG2b, κ

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 20 µl

Entrez Gene ID: 3577

RRID: AB_398489

Storage Buffer: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
An isotype control should be used at the same concentration as the antibody of interest.

関連製品

APC Mouse IgG2b κ Isotype Control RUO

サイズ 100 Tests カタログ番号 555745

551080 Rev. 3

抗体の詳細

5A12

Reacts with Interleukin-8 receptor A (IL-8RA), a G protein-linked seven transmembrane domain protein, expressed on monocytes and granulocytes. It is also expressed on a subset of T cells. Both IL-8RA and IL-8RB are high affinity receptors for IL-8. IL-8 is an inflammatory cytokine which induces chemotaxis of neutrophils, basophils and T lymphocytes. IL-8RA and IL-8RB were first clustered as CD128a and CD128b, respectively in the VIIth HLDA workshop, and then were re-clustered as CD181 and CD182, respectively in the VIIIth HLDA workshop.

551080 Rev. 3

フォーマットの詳細

APC

Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: APC

励起源: Red 627-640 nm

最大励起波長: 651 nm

最大蛍光波長: 660 nm

551080 Rev.3

引用＆参考文献

Development References (5)

Baggiolini M, Loetscher P, Moser B. Interleukin-8 and the chemokine family. Int J Immunopharmacol. 1995; 17(2):103-108. (Biology). View Reference
Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
Callard R, Gearing A. Callard R, Gearing A. The Cytokine Facts Book. San Diego: Academic Press; 1994.
Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002.
Qin S, LaRosa G, Campbell JJ, et al. Expression of monocyte chemoattractant protein-1 and interleukin-8 receptors on subsets of T cells: correlation with transendothelial chemotactic potential. Eur J Immunol. 1996; 26(3):640-647. (Clone-specific). View Reference

すべて表示する (5)

551080 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.