BD Pharmingen™ Purified Mouse Anti-Human PARP with Control
(RUO)
Western blot analysis of PARP. Control (lanes 1-3) and treated Jurkat lysates (lanes 4-6) were probed with anti-PARP (clone 7D3-6, Comp. No. 51-6639GR) at concentrations of 2.0 (lanes 1, 4), 1.0 (lanes 2, 5), and 0.5 µg/ml (lanes 3, 6). Intact PARP is identified as a band of 116 kDa, and cleaved PARP as a band of ~85 kDa.
Western blot analysis of PARP. Control (lanes 1-3) and treated Jurkat lysates (lanes 4-6) were probed with anti-PARP (clone 7D3-6, Comp. No. 51-6639GR) at concentrations of 2.0 (lanes 1, 4), 1.0 (lanes 2, 5), and 0.5 µg/ml (lanes 3, 6). Intact PARP is identified as a band of 116 kDa, and cleaved PARP as a band of ~85 kDa.
製品詳細
BD Pharmingen™
Western blot (Routinely Tested), Immunoprecipitation (Tested During Development), Flow cytometry, Functional assay (Reported)
RUO
AB_394009
調製と保管
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
推奨アッセイ手順
Jurkat control lysate [50 µg (1 µg/µl)] is provided as a western blot positive control (Comp. No. 51-16526N; store lysate at -20°C). Additional control lysate is sold separately.
製品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
551025 Rev. 4
構成品
| 説明 | 数量/容量 | Part Number | EntrezGene ID |
|---|---|---|---|
| Purified Mouse Anti-Human PARP | 50 µg (3 ea) | 51-6639GR | N/A |
| Jurkat Cell Lysate | 50 µg (1 ea) | 51-16526N | N/A |
551025 Rev. 4
引用＆参考文献
開発者向け参考資料 (2)
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Patel T, Gores GJ, Kaufmann SH. The role of proteases during apoptosis. FASEB J. 1996; 10(5):587-597. (Biology). 参考文献を見る
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Ranjit GB, Cheng MF, Mackay W, Whitacre CM, Berger JS, Berger NA. Poly(adenosine diphosphoribose) polymerase in peripheral blood leukocytes from normal donors and patients with malignancies. Clin Cancer Res. 1995; 1(2):223-234. (Immunogen: Dot Blot, Flow cytometry, Western blot). 参考文献を見る
551025 Rev. 4
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.