BD Pharmingen™ Purified Mouse Anti-Human PARP with Control
(RUO)
Western blot analysis of PARP. Control (lanes 1-3) and treated Jurkat lysates (lanes 4-6) were probed with anti-PARP (clone 7D3-6, Comp. No. 51-6639GR) at concentrations of 2.0 (lanes 1, 4), 1.0 (lanes 2, 5), and 0.5 µg/ml (lanes 3, 6). Intact PARP is identified as a band of 116 kDa, and cleaved PARP as a band of ~85 kDa.
Western blot analysis of PARP. Control (lanes 1-3) and treated Jurkat lysates (lanes 4-6) were probed with anti-PARP (clone 7D3-6, Comp. No. 51-6639GR) at concentrations of 2.0 (lanes 1, 4), 1.0 (lanes 2, 5), and 0.5 µg/ml (lanes 3, 6). Intact PARP is identified as a band of 116 kDa, and cleaved PARP as a band of ~85 kDa.
製品詳細
BD Pharmingen™
Western blot (Routinely Tested), Immunoprecipitation (Tested During Development), Flow cytometry, Functional assay (Reported)
RUO
AB_394009
調製と保管
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
推奨アッセイ手順
Jurkat control lysate [50 µg (1 µg/µl)] is provided as a western blot positive control (Comp. No. 51-16526N; store lysate at -20°C). Additional control lysate is sold separately.
製品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
551024 Rev. 5
構成品
| 説明 | 数量/容量 | Part Number | EntrezGene ID |
|---|---|---|---|
| Purified Mouse Anti-Human PARP | 50 µg (3 ea) | 51-6639GR | N/A |
| Jurkat Cell Lysate | 50 µg (1 ea) | 51-16526N | N/A |
551024 Rev. 5
引用＆参考文献
開発者向け参考資料 (2)
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Patel T, Gores GJ, Kaufmann SH. The role of proteases during apoptosis. FASEB J. 1996; 10(5):587-597. (Biology). 参考文献を見る
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Ranjit GB, Cheng MF, Mackay W, Whitacre CM, Berger JS, Berger NA. Poly(adenosine diphosphoribose) polymerase in peripheral blood leukocytes from normal donors and patients with malignancies. Clin Cancer Res. 1995; 1(2):223-234. (Immunogen: Dot Blot, Flow cytometry, Western blot). 参考文献を見る
551024 Rev. 5
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
