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BD Pharmingen™ Ig Isotype Control Cocktail - A
(RUO)
Regulatory Statusの凡例
Becton, Dickinson and Companyの書面による明示的な許諾を得た使用以外での製品の使用は固く禁じられています。
説明
The Isotype Control Cocktail A is a mixture of Mouse IgG1 and Mouse IgM designed and optimized to work in conjunction with the Rat T/B/NK Lymphocyte Cocktail (Cat. No. 558494). The mouse IgM G155-228 is a monoclonal antibody specific for trinitrophenol (TNP), a hapten not expressed on mammalian cells. The mouse IgG1 MOPC-21 immunoglobulin is a mouse myeloma protein. All immunoglobulins were selected as isotype controls following screening for low background on a variety of mouse and human tissues.
調製と保管
推奨アッセイ手順
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
製品通知
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
説明 | 数量/容量 | Part Number | EntrezGene ID |
---|---|---|---|
FITC mIgG1 | N/A | 51-540-20604 | N/A |
APC mIgM | N/A | 51-590-03089 | N/A |
PE mIgG1 | N/A | 51-550-33815 | N/A |
開発者向け参考資料 (1)
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Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology). 参考文献を見る
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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