Skip to main content Skip to navigation
PerCP Mouse Anti-Human CD8
製品詳細
Down Arrow Up Arrow


BD™
CD8α; CD8A; CD8 alpha; Leu2a; MAL; T8; p32
Human
Mouse BALB/c IgG1, κ
Human Peripheral Blood T Cells
Flow cytometry
6.25 μg/mL
20 μL
I T51,74; III T118,152,571
925
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation and Storage

Store vials at 2°C to 8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

347314 Rev. 1
抗体の詳細
Down Arrow Up Arrow
SK1

The CD8 antibody, clone SK1, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes.

The CD8 antibody recognizes the 32-kilodalton (kDa) α-subunit of a disulfide-linked bimolecular complex. The majority of peripheral blood CD8+ T lymphocytes expresses an α/β heterodimer (Mr 32, 30 kDa), while CD8+CD16+ natural killer (NK) lymphocytes and CD8+ T-cell receptor (TCR)-γ/δ+ T lymphocytes express an α/α homodimer (Mr 30 kDa). CD8+TCR-α/β+ T lymphocytes can express either an α/α homodimer or α/β heterodimer. The CD8 antigenic determinant binds to class I major histocompatibility (MHC) molecules, resulting in increased adhesion between the CD8+ T lymphocytes and target cells. Binding of the CD8 antigen to class I MHC molecules enhances the activation of resting T lymphocytes. The CD8 antigen is coupled to a protein tyrosine kinase, p56lck. The CD8:p56lck complex can play a role in T-lymphocyte activation through mediation of the interactions between the CD8 antigen and the CD3/TCR complex.

347314 Rev. 1
フォーマットの詳細
Down Arrow Up Arrow
PerCP
PerCP dye is part of the BD blue family of dyes. This dye is a fluorescent protein complex with an excitation maximum (Ex Max) of 481 nm and an emission maximum (Em Max) at 675 nm. PerCP is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405 nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PerCP
Blue 488 nm
481 nm
675 nm
347314 Rev.1
引用&参考文献
Down Arrow Up Arrow

Development References (24)

  1. Anderson P, Blue ML, Morimoto C, Schlossman SF. Cross-linking of T3 (CD3) with T4 (CD4) enhances the proliferation of resting T lymphocytes. J Immunol. 1987; 139:678-682. (Biology).
  2. Bernard A, Boumsell L, Hill C. Joint report of the first international workshop on human leucocyte differentiation antigens by the investigators of the participating laboratories. In: Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman SF, ed. Leucocyte Typing. New York, NY: Springer-Verlag; 1984:9-108.
  3. Campbell MJ, Scott J, Maecker HT, Park JW, Esserman LJ. Immune dysfunction and micrometastases in women with breast cancer. Breast Cancer Res Treat. 2005; 91:163-171. (Biology).
  4. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  5. Clinical and Laboratory Standards Institute. 2005. (Biology).
  6. Cosimi AB. Anti-T-cell monoclonal antibodies in transplantation therapy. Trans Proc. 1983; XV:1889-1892. (Biology).
  7. Eichmann K, Johnson J, Falk I, Emmrich F. Effective activation of resting mouse T lymphocytes by cross-linking submitogenic concentrations of the T-cell antigen receptor with either Lyt-2 or L3T4. Eur J Immunol. 1987; 17:643-650. (Biology).
  8. Engleman EG, Benike CJ, Evans RL. Circulating antigen-specific suppressor T cells in a healthy woman: mechanism of action and isolation with a monoclonal antibody. Clin Res. 1981; 29:365A. (Biology).
  9. Engleman EG, Benike CJ, Glickman E, Evans RL. Antibodies to membrane structures that distinguish suppressor/cytotoxic and helper T lymphocyte subpopulations block the mixed leukocyte reaction in man. J Exp Med. 1981; 154(1):193-198. (Biology). View Reference
  10. Evans RL, Wall DW, Platsoucas CD, et al. Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen. Proc Natl Acad Sci U S A. 1981; 78(1):544-548. (Biology). View Reference
  11. Gallagher PF, Fazekas de St. Groth B, Miller JFAP. CD4 and CD8 molecules can physically associate with the same T-cell receptor. Proc Natl Acad Sci USA. 1989; 86:10044-10048. (Biology).
  12. Gatenby PA, Kansas GS, Xian CY, Evans RL, Engleman EG. Dissection of immunoregulatory subpopulations of T lymphocytes within the helper and suppressor sublineages in man. J Immunol. 1982; 129(5):1997-2000. (Biology). View Reference
  13. Jacobson MA, Maecker HT, Orr PL, et al. Results of a cytomegalovirus (CMV)-specific CD8+/interferon-γ+ cytokine flow cytometry assay correlate with clinical evidence of protective immunity in patients with AIDS and CMV retinitis. J Infec Dis. 2004; 189:1362-1373. (Biology).
  14. Kotzin BL, Benike CJ, Engleman EG. Induction of immunoglobulin-secreting cells in the allogeneic mixed leukocyte reaction: regulation by helper and suppressor lymphocyte subsets in man. J Immunol. 1981; 127(9):931-935. (Biology). View Reference
  15. Lanier LL, Le AM, Phillips JH, Warner NL, Babcock GF. Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. J Immunol. 1983; 131(4):1789-1796. (Biology). View Reference
  16. Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-323. (Biology). View Reference
  17. Ledbetter JA, Frankel AE, Herzenberg. Human Leu T-cell differentiation antigens: quantitative expression on normal lymphoid cells and cell lines. In: Hammerling G, Hammerling U, Kearney J, ed. Monoclonal Antibodies and T Cell Hybridomas: Perspectives and Technical News. New York: Elsevier/North Holland Biomedical Press; 1981:16-22.
  18. Maecker HT, Maino VC. Analyzing T-cell responses to cytomegalovirus by cytokine flow cytometry. Hum Immunol. 2004; 65:493-499. (Biology).
  19. Moebius U. Knapp W, Dörken B, Gilks W, et al, ed. Leucocyte Typing IV. White Cell Differentiation Antigens. New York: Oxford University Press; 1989:342-343.
  20. Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). View Reference
  21. Rudd CE, Burgess KE, Barber EK, Schlossman SF. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:326-327.
  22. Suni MA, Ghanekar SA, Houck DW, et al. CD4+CD8dim T lymphocytes exhibit enhanced cytokine expression, proliferation and cytotoxic activity in response to HCMV and HIV-1 antigens. Eur J Immunol. 2001; 31:2512-2520. (Biology).
  23. Terry LA, DiSanto JP, Small TN, Flomenberg N. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:345-346.
  24. Tu W, Chen S, Sharp M, et al. Persistent and selective deficiency of CD4+T cell immunity to cytomegalovirus in immunocompetent young children. J Immunol. 2004; 172:3260-3267. (Biology).
すべて表示する (24) 表示項目を減らす
347314 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

 

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.