Preparation and Storage
The monoclonal antibody is supplied as 12 µg purified immunoglobulin in 2.0 mL (6 µg/mL) of phosphate-buffered saline (PBS). The FITC conjugate is supplied as 25 µg in 2.0 mL (12.5 µg/mL). See vial label for PerCP concentration. PBS contains gelatin and 0.1% sodium azide.The vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
CD61, clone RUU-PL 7F12, is derived from hybridization of mouse P3X63.Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with purified platelet membrane glycoproteins. CD61 recognizes an Mr 110-kdalton protein, also known as gpIIIa, the common b subunit (integrin b3-chain) of the gpIIb/IIIa complex and the vitronectin receptor (VNR).
Development References (13)
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Modderman PW. CD29/CDw49, CD47, CD51, CD55, and CD61. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1017-1019.
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Modderman PW. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:1025.
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Parmentier S, Kaplan C, Catimel B, McGregor JL. New families of adhesion molecules play a vital role in platelet functions.. Immunol Today. 1990; 11(7):225-7. (Biology). View Reference
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Shattil SJ, Hoxie JA, Cunningham M, Brass LF. Changes in the platelet membrane glycoprotein IIb.IIIa complex during platelet activation.. J Biol Chem. 1985; 260(20):11107-14. (Biology). View Reference
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Wong DA, Springer TA. Schlossman SF, Boumsell L, Gilks W, et al, ed. Leucocyte Typing V: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1995:1664-1665.
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von dem Borne AEGKr, Modderman PW, Admiraal LG, Nieuwenhuis, HK. Platelet antibodies, the overall results. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:951-966.
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von dem Borne AEGKr, Modderman PW. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:997-999.
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