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PE Mouse Anti-Human CD134
製品詳細
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BD™
OX40; OX 40; OX40L Receptor; TNFRSF4; ACT35; TXGP1L; OX40L Receptor; IMD16
Human
Mouse BALB/c IgG1, κ
Phytohemagglutinin-activated T lymphocytes
Flow cytometry
20 μL
7293
AB_400027
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation and Storage

The PE conjugate is supplied in 1.0 mL of PBS containing gelatin and 0.1% sodium azide. You may wish to titer the reagent for use in your specific application. Refer to the vial label for antibody concentration. The vial should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340420 Rev. 1
抗体の詳細
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L106

CD134, clone L106, is derived from hybridization of mouse Sp2/0 cells with spleen cells from BALB/c mice immunized with phytohemagglutinin-activated T lymphocytes.

CD134 (Anti-Human Ox 40) recognizes a 50-kdalton (kDa) antigen that is present on activated T lymphocytes. By sequence analysis, it is related to the rat monoclonal antibody Ox 40, a member of the nerve growth factor superfamily that also includes CD27, CD30, CD40, CD95 (Fas), and the tumor necrosis factors alpha and beta (CD120a and CD120b).

340420 Rev. 1
フォーマットの詳細
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
340420 Rev.1
引用&参考文献
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Development References (7)

  1. Barclay AN, Birkeland ML, Brown MH, et al. 1993.
  2. Fanslow WC, Clifford KN, Zappone J, Sorensen R, Gaum P. Involvement of Ox40 interaction in murine cardiac allograft rejection. 1995; 575a. (Biology).
  3. Godfrey W, Buck D, Harara M, Benike C, Engleman E. Studies of the L106 protein and its gene: Evidence that L106 and Ox 40 are homologous. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1157-1160.
  4. Godfrey WR, Fagnoni FF, Harara MA, Buck D, Engleman EG. Identification of a human OX-40 ligand, a costimulator of CD4+ T cells with homology to tumor necrosis factor. J Exp Med. 1994 August; 180(2):757-762. (Biology). View Reference
  5. Mallett S, Fossum S, Barclay AN. Characterization of the MRC OX40 antigen of activated CD4 positive T lymphocytes--a molecule related to nerve growth factor receptor. EMBO J. 1990; 9(4):1063-1068. (Biology). View Reference
  6. Paterson DJ, Jefferies WA, Green JR, et al. Antigens of activated rat lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T cell blasts. Mol Immunol. 1993; 24:1282. (Biology).
  7. Tittle TV, Steinkeler C, Weinberg A, Maziarz R. The expression of the T cell activation antigen, Ox-40, in GVHD. Blood. 1995; 86(10):575a-576a. (Biology).
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340420 Rev. 1

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